Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology

作     者：LU Yan MEI Le-he 

作者机构：Department of Chemical and Biochemical Engineering Zhejiang University Hangzhou 310027 China 

基　　金：Project (No. 30570411) supported by the National Natural ScienceFoundation of China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2007年第8卷第1期

页      码：27-32页

摘      要：Factorial design and response surface techniques were used to design and optimize increasing P450 BM-3 expression in E, coll. Operational conditions for maximum production were determined with twelve parameters under consideration： the concentration of FeCl3, induction at OD578 （optical density measured at 578 nm）, induction time and inoculum concentration. Initially, Plackett-Burman （PB） design was used to evaluate the process variables relevant in relation to P450 BM-3 production. Four statistically significant parameters for response were selected and utilized in order to optimize the process. With the 416C model of hybrid design, response surfaces were generated, and P450 BM-3 production was improved to 57.90×10^-3 U/ml by the best combinations of the physicochemical parameters at optimum levels of 0,12 mg/L FeCl3, inoculum concentration of 2.10%, induction at OD578 equal to 1.07, and with 6.05 h of induction.

主 题 词：Optimization P450 BM-3 Plackett-Burman (PB) design Hybrid design 

学科分类：0710[理学-生物科学类] 1007[医学-药学类] 1004[医学-公共卫生预防医学类] 081703[081703] 1001[医学-基础医学] 0905[农学-林学类] 08[工学] 0817[工学-轻工类] 0906[农学-水产类] 0836[0836] 082203[082203] 0822[工学-核工程类] 

核心收录：

D　O　I：10.1631/jzus.2007.B0027

馆 藏 号：203101383...