Statistically designed enzymatic hydrolysis of an icariin/b-cyclodextrin inclusion complex optimized for production of icaritin

作     者：Xin Jin Zhenhai Zhang E.Suna Songlin Li Xiaobin Jia 

作者机构：Key Laboratory of New Drug Delivery System of Chinese Meteria MedicaJiangsu Provincial Academy of Chinese MedicineNanjing 210028China College of PharmacyNanjing University of Chinese MedicineNanjing 210046China 

基　　金：supported by the National Natural Science Foundation of China(No.30973944/H2805) the foundation for high-level talent on six areas of Jiangsu province 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2012年第2卷第1期

页      码：83-89页

摘      要：This study focuses on the preparation and enzymic hydrolysis of an icariin/bcyclodextrin inclusion complex to efficiently generate *** physical characteristics of the inclusion complex were evaluated by differential scanning calorimetry(DSC).Enzymatic hydrolysis was optimized for the conversion of icariin/b-cyclodextrin complex to icaritin by Box–Behnken statistical *** inclusion complex formulation increased the solubility of icariin approximately 17-fold,from 29.2 to 513.5 mg/mL at 60℃.The optimum conditions were predicted by Box–Behnken statistical design as follows:60℃,pH 7.0,the ratio of enzyme/substrate(1:1.1)and reaction time 7 *** the optimal conditions the conversion of icariin was 97.91%and the reaction time was decreased by 68%compared with that without b-CD *** analysis by melting point,ESI-MS,UV,IR,1H NMR and 13C NMR confirmed the authenticity of icaritin with a purity of 99.3%and a yield of 473 mg of icaritin from 1.1 g icariin.

主 题 词：Icariin/b-CD inclusion complex Snailase Enzymatic hydrolysis Box–Behnken statistical design Icaritin 

学科分类：081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 070303[070303] 0703[理学-化学类] 

D　O　I：10.1016/j.apsb.2011.12.004

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