A biomimetic basementmembrane consisted of hybrid aligned nanofibers andmicrofibers with immobilized collagen IV and laminin for rapid endothelialization

作     者：Chenglong Yu Guoping Guan Stefanie Glas Lu Wang Zhutong Li Lih-Sheng Turng Chenglong Yu;Guoping Guan;Stefanie Glas;Lu Wang;Zhutong Li;Lih-Sheng Turng

作者机构：Key Laboratory of Textile Industry for Biomedical Textile Materials and TechnologyCollege of TextilesDonghua University2999 North Renmin RoadSongjiang DistrictShanghai 201620China Engineering Research Center of Technical Textiles of Ministry of EducationCollege of TextilesDonghua UniversityShanghai 201620China Wisconsin Institute for DiscoveryUniversity of Wisconsin-Madison330 North Orchard StreetMadisonWI 53715USA Department of Mechanical EngineeringUniversity of Wisconsin-Madison1513 University AveMadisonWI 53706USA 

基　　金：This work was supported by the Fundamental Research Funds for the Central Universities(Nos.2232019G-06 and 2232019A3-06) 111 project(No.PB0719035) The authors at University of Wisconsin-Madison would like to acknowledge the partial support by the Wisconsin Institute for Discovery(WID),the NHLBI of the National Institutes of Health(No.U01HL134655) the Kuo K.and Cindy F.Wang Professorship.Chenglong Yu also acknowledged the fellowship from the China Scholarship Council(CSC)under the Grant CSC No.201906630070 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2021年第4卷第2期

页      码：171-189页

摘      要：Rapid formation of a continuous endothelial cell(EC)monolayer with healthy endothelium function on the luminal surface of vascular implants is imperative to improve the longtime patency of small-diameter vascular *** the present study,we combined the contact guidance effects of aligned nanofibers,which enhance EC adhesion and proliferation because of its similar fiber scale with native vascular basement membranes,and aligned microfibers,which could induce EC elongation effectively and allow ECs *** was followed by successive immobilization of collagen IV and laminin to fabricate a biomimetic basement membrane(BBM)with structural and compositional *** hemolysis assay and platelet adhesion results showed that the BBM exhibited excellent ***,the adhered human umbilical vein endothelial cells(HUVECs)onto theBBMaligned along the orientation of the microfibers with an elongated morphology,and the data demonstrated that the BBM showed favorable effects on EC attachment,proliferation,and *** oriented EC monolayer formed on the BBM exhibited improved antithrombotic capability as indicated by higher production of nitric oxide and prostacyclin(PGI2).Furthermore,fluorescence images indicated that HUVECs could infiltrate into the BBM,implying theBBM’s ability to enhance transmural ***,theBBMpossessed the properties to regulate ECbehaviors and allow transmural ingrowth,demonstrating the potential to be applied as the luminal surface of small-diameter vascular implants for rapid endothelialization.

主 题 词：Biomimetic basement membranes Aligned electrospun fibers Surface modification Endothelialization Anti-thrombogenicity Transmural ingrowth 

学科分类：0710[理学-生物科学类] 0831[工学-公安技术类] 0817[工学-轻工类] 08[工学] 0807[工学-电子信息类] 080501[080501] 0805[工学-能源动力学] 080502[080502] 0802[工学-机械学] 0836[0836] 0811[工学-水利类] 

核心收录：

D　O　I：10.1007/s42242-020-00111-6

馆 藏 号：203101671...