Optimization of the Technique for Liquid Culture of Pleurotus cornucopiae Seeds

作     者：温以斌 吴义飞 王多明 李冠喜 刘辉 刘耀鸿 Yibin WEN;Yifei WU;Duoming WANG;Guanxi LI;Hui LIU;Yaohong LIU

作者机构：连云港市农业科学院江苏连云港222006 南京林业大学森林资源与环境学院江苏南京210037 江苏省入侵有害生物预防与控制重点实验室江苏南京210037 

基　　金：Supported by the Agricultural "Three Terms" Program in Jiangsu Province (sx (2009) 133) the Plan Program for Demonstration of Scientific and Technological Achievements in Agriculture of Lianyungang City(SF0902) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2013年第14卷第3期

页      码：507-511页

摘      要：[Objective] This study aimed to optimize the conditions for liquid culture of P. comucopiaeseeds. [Method] ＂P. cornucopiae 2＇ selected by our laboratory was used as the experimental strain in this study. Single factor experiment in conjunction with Plackett-Burman （PB） design as well as Response Surface Methodology（RSM） based on Central Composite Design （CCD） were used to screen and optimize the eight factors influencing the technique for liquid culture of P. cornucopiae seeds. [Result] Firstly, the eight factors influencing the biomass of P. cornucopiae seeds in liquid culture were confirmed by means of single factor experiment; secondly, through Plackett-Burman experiment design, three main factors influencing the biomass of P. cornucopiae seeds in liquid culture were screened, namely the amount of added mannitol, the initial pH and culture temperature; lastly, the second order model, that is Y=-70.72＋2.44X3 ＋2.67X7 ＋3.72X8 ＋0.08X3 X7 -0.01X7 X8-0.05X32- 0.34X72-0.08X82, was obtained through Central Composite Design （CCD） along with Response Surface Methodology （RSM）. [Conclusion] The optimal conditions for the production of P. cornucopiae seeds in liquid culture were as follows： the amount of added mannitol of 28.17 g/L, the initial pH controlled at 5.90, 250 ml erlenmeyer flask filled with 100 ml of liquid medium, inoculum size of 5%（v/v）, rotation speed of 150 r/min, and gyratory culture at 23.97℃ for 5 days.

主 题 词：Pleurotus cornucopiae Biomass Influencing factors Optimization 

学科分类：09[农学] 0902[农学-自然保护与环境生态类] 090202[090202] 

D　O　I：10.3969/j.issn.1009-4229(e).2013.03.026

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