Porous hydrogel arrays for hepatoma cell spheroid formation and drug resistance investigation

作     者：Xin Lei Changmin Shao Xin Shou Keqing Shi Liang Shi Yuanjin Zhao Xin Lei;Changmin Shao;Xin Shou;Keqing Shi;Liang Shi;Yuanjin Zhao

作者机构：Translational Medicine LaboratoryThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035China Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001China State Key Laboratory of BioelectronicsSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Department of Laboratory MedicineThe Eighth Affiliated HospitalSun Yat-Sen UniversityShenzhen 518033China 

基　　金：supported by the National Key Research and Development Program of China(No.2020YFA0908200) the National Natural Science Foundation of China(Nos.52073060,61927805,81974312,and 81501823) the Natural Science Foundation of Jiangsu Province(No.BE2018707) the Shenzhen Fundamental Research Program(No.JCYJ20190813152616459) the Zhejiang Provincial Natural Science Foundation of China(Nos.LY18H160049 and LQ19H160008) the Medical Scientific Research of Zhejiang Province(No.2017KY459) Wenzhou Municipal Science and Technology Bureau(No.Y20190203) Wenzhou Institute,University of Chinese Academy of Sciences’s startup fund(No.WIUCASQD2019007) 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2021年第4卷第4期

页      码：842-850页

摘      要：Drug resistance is one of the major obstacles in the drug therapy of *** in this area in pre-clinical research have focused on developing novel platforms to evaluate and decrease drug *** this paper,inspired by the structure of hives where swarms live and breed,we propose porous hydrogel arrays with a uniform pore structure for the generation of hepatoma cell spheroids and the investigation of drug *** porous hydrogel arrays were fabricated using polyeth-ylene glycol diacrylate(PEGDA)hydrogel to negatively replicate a well-designed *** from the elaborate processing of the template,the prepared porous hydrogel arrays possessed a uniform pore *** to their anti-adhesion properties and the excellent biocompatibility of the PEGDA hydrogel,the hepatoma cells could form well-defined and uni-form hepatoma cell spheroids in the porous hydrogel *** found that the resistant hepatoma cell spheroids showed more significant Lenvatinib resistance and a migratory phenotype compared with a two-dimensional(2D)cell culture,which reveals the reason for the failure of most 2D cell-selected drugs for in vivo *** features give such porous hydrogel arrays promising application prospects in the investigation of tumor cell spheroid culture and in vitro drug resistance.

主 题 词：Drug resistance Hydrogel Porous Spheroid Hepatoma 

学科分类：0710[理学-生物科学类] 0831[工学-公安技术类] 1002[医学-临床医学类] 0817[工学-轻工类] 0807[工学-电子信息类] 0805[工学-能源动力学] 0802[工学-机械学] 100214[100214] 0836[0836] 0811[工学-水利类] 10[医学] 

核心收录：

D　O　I：10.1007/s42242-021-00141-8

馆 藏 号：203104265...