CLONING AND EXPRESSION OF SPODOPTERA LITURA UBIQUITIN GENE

作     者：李朝飞 龚映雪 师永霞 潘丽晶 庞义 

作者机构：中山大学生物防治国家重点实验室广州510275 

基　　金：国家重大基础研究项目 (973 ) (No .G2 0 0 0 0 1 62 0 9) 国家自然科学基金项目 (No.3 0 1 70 0 40 )资助 

出 版 物：《Entomologia Sinica》 (中国昆虫科学（英文版）)

年 卷 期：2003年第10卷第1期

页      码：27-34页

摘      要：Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF436066). Th e le ngth of this ORF is 228bp, encoding a protein with Mr of 8.56 kD and isoelectri c point of 6.56. Multiple sequence alignment indicated that *** UBI is very similar to the homologous proteins of other eukaryotic species and it has 84% id entity with *** nucleopolyhedrovirus (SpltMNPV) UBI at amino acid level . RT -PCR analysis showed that *** UBI gene is ubiquitously expressed in la rva t issues which are susceptible to SpltMNPV infection. By constructing *** e xpre ssion vector, *** UBI was highly expressed and the recombinant protein was purified using Ni-NTA resin column, and currently further study on the function of *** UBI in SpltMNPV infection is underway.

主 题 词：Spodoptera litura ubiquitin gene cloning gene expressi on 

学科分类：0710[理学-生物科学类] 07[理学] 08[工学] 09[农学] 071007[071007] 0901[农学-植物生产类] 0836[0836] 090102[090102] 

核心收录：

D　O　I：10.1111/j.1744-7917.2003.tb00361.x

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