A simple,rapid,and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides

作     者：Siqi Li Kaichan Su Zhenkun Zhuang Qing Qin Lei Gao Yamei Deng Xuyang Liu Guixue Hou Longtao Wang Piliang Hao Huanming Yang Siqi Liu Hongming Zhu Yan Ren 李思奇;苏恺婵;庄镇堃;秦晴;高磊;邓亚美;刘旭阳;侯桂雪;王龙涛;郝丕良;杨焕明;刘斯奇;朱鸿明;任艳

作者机构：BGI-ShenzhenShenzhen 528083China Department of BiologyUniversity of CopenhagenCopenhagen 2100Denmark BGI-Wuhan Clinical LaboratoriesBGI-ShenzhenWuhan 430000China Department of CardiologyZhongshan HospitalFudan UniversityShanghai Institute of Cardiovascular DiseaseShanghai 200032China Department of Cardiovascular SurgeryThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of China(USTC)Hefei 230001China School of Life Science and TechnologyShanghai Tech UniversityShanghai 201210China Institute for Regenerative MedicineShanghai East HospitalTongji University School of MedicineShanghai 200120China Experiment Center for Science and TechnologyShanghai University of Traditional Chinese MedicineShanghai 201203China 

基　　金：supported by the National Key R&D Program of China(2020YFE0202200) the National Natural Science Foundation of China(82070365) the Basic Research General Program of Shenzhen(JCYJ20190807103407873) 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2022年第67卷第6期

页      码：581-584,M0003页

摘      要：Since a typical mammalian cell contains only 200–300 picograms of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell proteomics has long been awaited.A series of methods have been explored to make single-cell proteomics *** design of nanoPOTS and OAD chip-based processes minimizes sample preparation steps and volumes to reduce sample loss[2,3].

主 题 词：乙醛脱氢酶2 单细胞蛋白质 蛋白质组学 潜在作用 质谱数据 数据采集模式 HeLa细胞 快速检测方法 

学科分类：0710[理学-生物科学类] 07[理学] 071009[071009] 09[农学] 0901[农学-植物生产类] 090102[090102] 

核心收录：

D　O　I：10.1016/j.scib.2021.12.022

馆 藏 号：203109033...