Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

作     者：Rubén Mateos-Fernández Elena Moreno-Giménez Silvia Gianoglio Alfredo Quijano-Rubio Jose Gavaldá-García Lucía Estellés Alba Rubert JoséLuis Rambla Marta Vazquez-Vilar Estefanía Huet Asunción Fernández-del-Carmen Ana Espinosa-Ruiz Mojca Juteršek Sandra Vacas Ismael Navarro Vicente Navarro-Llopis Jaime Primo Diego Orzáez 

作者机构：Institute for Plant Molecular and Cell Biology(IBMCP)Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Politécnica de Valencia(UPV)ValenciaSpain Jaume I UniversityCastellon de la PlanaSpain Department of Biotechnology and Systems BiologyNational Institute of BiologyLjubljanaSlovenia Jožef Stefan International Postgraduate SchoolLjubljanaSlovenia Centro de Ecología Química AgrícolaInstituto Agroforestal del MediterráneoUniversitat Politècnica de ValènciaValenciaSpain Ecología Y Protección Agrícola SLValenciaSpain 

基　　金：support of the UPV Generación Espontánea program.Rubén Mateos-Fernández acknowledges support by a PhD grant(ACIF/2019/226)from Generalitat Valenciana.Elena Moreno-Giménez acknowledges support by a PhD grant(FPU18/02019)from the Spanish Ministry of Science,Innovation and Universities.JoséL.Rambla acknowledges support by a“Juan de la Cierva-Formación”grant(FJCI-2016-28601)from the Spanish Ministry of Economy and Competitiveness Marta Vazquez-Vilar is a recipient of APOSTD/2020/096(Generalitat Valenciana and Fondo Social Europeo postdoctoral grant) This work was funded by Era-CoBiotech SUSPHIRE(PCI2018-092893)grants 

出 版 物：《BioDesign Research》 (生物设计研究（英文）)

年 卷 期：2021年第2021卷第1期

页      码：79-95页

摘      要：Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in ***,we describe the engineering of transgenic plants producing(Z)-11-hexadecenol(Z11-16OH)and(Z)-11-hexadecenyl acetate(Z11-16OAc),two main volatile components in many Lepidoptera sex pheromone *** assembled multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them into Nicotiana benthamiana *** constructs contained the Amyelois transitella AtrΔ11 desaturase gene,the Helicoverpa armigera fatty acyl reductase HarFAR gene,and the Euonymus alatus diacylglycerol acetyltransferase EaDAct gene in different *** the pheromone-producing plants showed dwarf phenotypes,the severity of which correlated with pheromone *** but one of the recovered lines produced high levels of Z11-16OH,but very low levels of Z11-16OAc,probably as a result of recurrent truncations at the level of the EaDAct *** one plant line(SxPv1.2)was recovered that harboured an intact pheromone pathway and which produced moderate levels of Z11-16OAc(11.8μg g^(-1) FW)and high levels of Z11-16OH(111.4μg g^(-1)).Z11-16OAc production was accompanied in SxPv1.2 by a partial recovery of the dwarf ***1.2 was used to estimate the rates of volatile pheromone release,which resulted in 8.48 ng g-1 FW per day for Z11-16OH and 9.44 ng g^(-1 )FW per day for *** results suggest that pheromone release acts as a limiting factor in pheromone biodispenser strategies and establish a roadmap for biotechnological improvements.

主 题 词：release dwarf transformed 

学科分类：0202[经济学-财政学类] 02[经济学] 020205[020205] 

D　O　I：10.34133/2021/9891082

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