Toward modular construction of cell‐free multienzyme systems

作     者：Yinchen Zhang Ning Nie Yifei Zhang 张寅晨;聂宁;张一飞

作者机构：State Key Laboratory of Chemical Resource EngineeringBeijing University of Chemical TechnologyBeijing 100029China Beijing Advanced Innovation Center for Soft Matter Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China 

基　　金：国家重点研发计划(2021YFC2104100) 国家自然科学基金(NSFC-22178018) 

出 版 物：《Chinese Journal of Catalysis》 (催化学报（英文）)

年 卷 期：2022年第43卷第7期

页      码：1749-1760页

摘      要：The implementation of multiple enzymes for chemical production in a cell‐free scenario is an emerging field in *** enables the redesign and reconstitution of new enzymatic routes for producing chemicals that may be hard to obtain from natural *** the construction of a cell‐free multienzyme system is highly flexible and adaptable,it is challenging to make all enzymatic reactions act in ***,modular construction has been conceptual‐ized as an effective way to harmonize diverse enzymatic *** this review,we introduce the concept of a multienzyme module and exemplify representative modules found in *** then categorize recent developments of synthetic multienzyme modules into main‐reaction modules and auxiliary modules according to their roles in reaction *** highlight four main‐reaction mod‐ules that can perform carbon metabolism,carbon assimilation,protein glycosylation and nonribo‐somal peptide synthesis,and exemplify auxiliary modules used for energy supply,protection and reinforcement for main *** reactor‐level modularization of multienzyme catalysis is also discussed.

主 题 词：Cell-free biosynthesis In vitro synthetic biology Multienzyme system Modular construction Synthetic module 

学科分类：081705[081705] 08[工学] 0817[工学-轻工类] 080502[080502] 0805[工学-能源动力学] 

核心收录：

D　O　I：10.1016/S1872-2067(21)64002-0

馆 藏 号：203111760...