Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design

作     者：Leticia I. RAMíREZ-CAVAZOS Charles JUNGHANNS Rakesh NAIR Diana L. CáRDENAS-CHáVEZ Carlos HERNáNDEZ-LUNA Spiros N. AGATHOS Roberto PARRA 

作者机构：Centro del Agua para América Latina y el CaribeTecnológico de MonterreyCampus MonterreyNL 64849Mexico Helmholtz Centre for Environmental Research-UFZPermoserstra?e 1504318 LeipzigGermany Bioengineering GroupEarth and Life InstituteUniversité Catholique de LouvainPlace Croix du Sud2Box L7.05.191348 Louvain-la-NeuveBelgium Laboratorio de EnzimologíaFacultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónAv. Universidad s/nCiudad UniversitariaSan Nicolás de los GarzaNL 64450Mexico 

基　　金：supported by the National Science and Technology Council of Mexico(CONACYT)(No.192118) 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2014年第15卷第4期

页      码：343-352页

摘      要：The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate（CuSO4）.The best conditions obtained from screening experiments in shaken flasks using tomato juice,CuSO4,and soybean oil were integrated in an experimental *** levels of tomato juice as the medium,CuSO4and soybean oil as inducers（36.8%（v/v）,3 mmol/L,and 1%（v/v）,respectively） were determined for 10 L stirred tank bioreactor *** combination resulted in laccase titer of 143000 IU/L（2,2＇-azino-bis（3-ethylbenzthiazoline-6- sulfonic acid）,pH 3.0）,which represents the highest activity so far reported for *** in a 10-L *** interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20000 IU/L,whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 *** on a partial characterization,the laccases of this strain are especially promising in terms of thermostability（half-life of 6.1 h at 60 °C） and activity titers.

主 题 词：Enhanced laccase production Central composite design Screening media Inducers Tomato juice medium Soybean oil Copper sulfate 

学科分类：0710[理学-生物科学类] 071010[071010] 07[理学] 

核心收录：

D　O　I：10.1631/jzus.B1300246

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