Microcapillary cell extrusion deposition with picolitre dispensing resolution

作     者：Saeed Fathi Iek Man Lei Yang Cao Yan Yan Shery Huang Saeed Fathi;Iek Man Lei;Yang Cao;Yan Yan Shery Huang

作者机构：Department of EngineeringUniversity of CambridgeCambridgeUK The Nanoscience CentreUniversity of CambridgeCambridgeUK 

基　　金：supported by the European Research Council(ERC-St G,758865,to YYSH) the financial support from the W.D.Armstrong Trust and the Macao Postgraduate Scholarship Fund 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第1期

页      码：1-11页

摘      要：Extrusion-based cell deposition has become a prominent technique for expanding bioprinting ***,the associated print resolution in the order of nanolitre or above has been a limiting *** demand for improving print resolution towards the scale of a single cell has driven the development of precision nozzle extrusion,although the benefits gained remain ***,aided by in situ imaging,we investigated the dynamics of cell organisation through an extrusion-based microcapillary tip with picolitre precision through in-air or immersion *** microcapillary extrusion setup,termed‘Picodis’,was demonstrated by generating droplets of colouring inks immersed in an immiscible ***,using 3T3 fibroblast cells as an experimental model,we demonstrated the deposition of cell suspension,and pre-aggregated cell ***,the dynamic organisation of cells within the microcapillary tip was described,along with cell ejection and deposition upon exiting the tip *** vision-assisted approach revealed that when dispersed in a culture medium,the movements of cells were distinctive based on the flow profiles and were purely driven by laminar fluid flow within a narrow *** primary process limitations were cell sedimentation,aggregation and compaction,along with trapped air *** use of picolitre-level resolution microcapillary extrusion,although it provides some level of control for a small number of cells,does not necessarily offer a reliable method when a specified number of cells are *** study provides insights into the process limitations of high-resolution cell ink extrusion,which may be useful for optimising biofabrication processes of cell-laden constructs for biomedical research.

主 题 词：Microcapillary cell printing Resolution dispensing Cell sedimentation Single cell 

学科分类：1001[医学-基础医学] 100102[100102] 10[医学] 

核心收录：

D　O　I：10.1007/s42242-022-00205-3

馆 藏 号：203114500...