Evaluation of novel decellularizing corneal stroma for cornea tissue engineering applications

作     者：Yi Shao, Lu Yang 

作者机构：Department of Ophthalmology the First Affiliated Hospital of Nanchang University Nanchang 330006 Jiangxi Province China Department of Endocrinology the Third Hospital of Nanchang the Affiliated Nanchang Hospital of Southern Medical University Nanchang 330009 Jiangxi Province China Department of Physiology Endocrinology Institute of Oklahoma University Oklahoma City OK 73104-5020 USA Department of Ophthalmology the First Affiliated Hospital of Nanhua University Hengyang 421001 Hunan Province China 

基　　金：National Natural Science Foundation of China (No.81160118,81100648,81101858,81100649) Natural Science Foundation of Jiangxi Province,China (No.20114BAB215029) Technology Foundation of Jiangxi Province,China (No.20111BBG70026-2) Health Department Science and Technology Foundation,China (No.20121026) Education Department Youth Scientific Research Foundation,China (No.JJJ12158) National High Technology Research of China (863 project)(No.2006AA02A131) 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2012年第5卷第4期

页      码：415-418页

摘      要：AIM:To develop a new decellularization method depended upon the natural corneal structure and to harvest an ideal scaffold with good biocompatibilities for corneal ***:The acellular cornea matrix (ACM) were prepared from de-epithelium fresh porcine corneas (DFPCs) by incubation with 100% fresh human sera and additional electrophoresis at 4℃. Human corneal epithelial cells (HCEs) were used for the cytotoxicity tests of ACM. ACM were implanted into the Enhanced Green Fluorecence Protein (eGFP) transgenic mouse anterior chamber for evaluation of ***:HE and GSIB4 results showed fresh porcine cornea matrix with 100% human sera and electrophoresis could entirely decellularize stromal cell without reducing its transparency. ACM has no cytotoxic effect ex vivo. Animal test showed there was no rejection for one month after ***:These results provide a decellularizing approach for the study of corneal tissue engineering and had the broader implications for the field of biological tissue engineering in other engineered organ or tissue matrix.

主 题 词：cornea human sera electrophoresis tissue engineering decellularization biocompatibility 

学科分类：08[工学] 080502[080502] 0805[工学-能源动力学] 

核心收录：

D　O　I：10.3980/j.issn.2222-3959.2012.04.02

馆 藏 号：203114622...