Stoichiometric Conversion of Maltose for Biomanufacturing by In Vitro Synthetic Enzymatic Biosystems

作     者：Guowei Li Xinlei Wei Ranran Wu Wei Zhou Yunjie Li Zhiguang Zhu Chun You 

作者机构：Tianjin Institute of Industrial BiotechnologyChinese Academy of Sciences32 West 7th AvenueTianjin Airport Economic AreaTianjin 300308China College of BiotechnologyTianjin University of Science and Technology1038 Dagu NanluHexi DistrictTianjin 300457China National Technology Innovation Center of Synthetic BiologyTianjin 300308China 

基　　金：the National Key Research and Development Program of China(Grant number 2021YFA0910601) the National Natural Science Foundation of China(Grant numbers 32022044 and 32001027) 

出 版 物：《BioDesign Research》 (生物设计研究（英文）)

年 卷 期：2022年第2022卷第1期

页      码：217-227页

摘      要：Maltose is a natural α-(1,4)-linked disaccharide with wide applications in food industries and microbial fermentation. However,maltose has scarcely been used for in vitro biosynthesis, possibly because its phosphorylation by maltose phosphorylase (MP)yields β-glucose 1-phosphate (β-G1P) that cannot be utilized by α-phosphoglucomutase (α-PGM) commonly found in in vitrosynthetic enzymatic biosystems previously constructed by our group. Herein, we designed an in vitro synthetic enzymaticreaction module comprised of MP, β-phosphoglucomutase (β-PGM), and polyphosphate glucokinase (PPGK) for thestoichiometric conversion of each maltose molecule to two glucose 6-phosphate (G6P) molecules. Based on this syntheticmodule, we further constructed two in vitro synthetic biosystems to produce bioelectricity and fructose 1,6-diphosphate (FDP),respectively. The 14-enzyme biobattery achieved a Faraday efficiency of 96.4% and a maximal power density of 0.6mW/cm^(2),whereas the 5-enzyme in vitro FDP-producing biosystem yielded 187.0mM FDP from 50 g/L (139mM) maltose by adopting afed-batch substrate feeding strategy. Our study not only suggests new application scenarios for maltose but also provides novelstrategies for the high-efficient production of bioelectricity and value-added biochemicals.

主 题 词：enzymatic utilized maximal 

学科分类：081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 070303[070303] 0703[理学-化学类] 0836[0836] 

D　O　I：10.34133/2022/9806749

馆 藏 号：203114653...