Alginate/gelatin/boron-doped hydroxyapatite-coated Ti implants:in vitro and in vivo evaluation of osseointegration
作者机构：Department of Engineering SciencesMiddle East Technical UniversityAnkaraTurkey Department of Histology and EmbryologyAfyon Kocatepe UniversityAfyonTurkey Department of SurgeryAfyon Kocatepe UniversityAfyonTurkey Center of Excellence in Biomaterials and Tissue EngineeringAnkaraTurkey
基 金：Acknowledgements Authors would like to thank Center of Excellence in Biomaterials and Tissue Engineering(BIOMATEN)for the support provided.Authors also acknowledge financial support provided by National Boron Institute(BOREN Grant No:2018-31-07-25-001)
出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)
年 卷 期：2023年第6卷第3期
页 码：217-242页
摘 要：In this study,boron-doped hydroxyapatite(BHT)-loaded alginate/gelatin-based(A/G)hydrogel coating on Ti was fabricated to support bone integration through triggering osteoinduction,vascularization and ***,highly reproducible,cheap and time-effective BHT was produced,which significantly promoted higher osteogenic and angiogenic maturation,while a mild innate immune response was *** immense potential of BHT was evidenced by the production of a gap-filling A/G/BHT interphase on Ti implants to mimic the osseous extracellular matrix to achieve functional bridging and exert control over the course of innate immune *** initially aminosilanized the implant surface using 3-aminopropyl triethoxysilane,and then coated it with 0.25%w/v alginate with 20 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide to allowthe A/G/BHT pre-gel to disperse evenly and covalently attach on the *** pre-gel was added with 0.2 M NaCl to homogeneously blend BHT in the structure without inducing ionic ***,the coated implants were freeze-dried and *** coated layer demonstrated high cohesive and adhesive strength,and 8-month-long shelf-life at room temperature and normal *** A/G/BHT was able to coat an irregularly shaped Ti *** and endothelial cells thrived on the A/G/BHT,and it demonstrated greatly improved osteogenic and angiogenic ***,A/G/BHT maintained macrophage viability and generated an acute increase in immune response that could be resolved ***,A/G/BHT was shown to induce the robust integration of implant in a rabbit femur osteochondral model within ***,we concluded that A/G/BHT coatings could serve as amultifunctional reservoir,promoting the strong and rapid osseointegration of metallic implants.
主 题 词：Boron Alginate/gelatin Implant coating Titanium Osteochondral model
学科分类：080503[080503] 1003[医学-口腔医学类] 08[工学] 0805[工学-能源动力学] 100302[100302] 10[医学]
核心收录：
D O I：10.1007/s42242-022-00218-y
馆 藏 号：203118245...
