Revealing CO_(2)-Fixing SAR11 Bacteria in the Ocean by Raman-Based Single-Cell Metabolic Profiling and Genomics

作     者：Xiaoyan Jing Yanhai Gong Teng Xu Paul ADavison Craig MacGregor-Chatwin CNeil Hunter La Xu Yu Meng Yuetong Ji Bo Ma Jian Xu Wei E.Huang 

作者机构：Single-Cell CenterCAS Key Laboratory of BiofuelsShandong Key Laboratory of Energy Genetics and Shandong Institute of Energy ResearchQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoShandongChina Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoShandongChina University of Chinese Academy of SciencesBeijingChina PlantsPhotosynthesis and SoilSchool of BiosciencesUniversity of SheffieldSheffield S102TNUK Disease and Fishery Drugs Research CenterMarine Biology Institute of Shandong ProvinceQingdaoShandongChina Single-Cell BiotechnologyLtdQingdaoShandongChina Department of Engineering ScienceUniversity of OxfordParks RoadOX13PJ OxfordUK 

基　　金：the National Key R&D Program Young Scientists Project of China(No.2021YFD1900400 to XYJ) the National Science Foundation of China(No.32270109 to XYJ) BM acknowledges the support of the Key Instrument Development Program from Chinese Academy of Sciences(YJKYYQ20170017) WEH acknowledges finance and instrumentation support from the Engineering and Physical Sciences Research Council(EP/M002403/1 and EP/M02833X/1)and Natural Environment Research Council(NE/M002934/1) WEH,PAD,CMC,and CNH acknowledge financial support from the Biotechnology and Biological Sciences Research Council(BBSRC UK),award number BB/M000265/1 CNH and PAD are supported by the European Research Council Synergy Award 854126.We also thank NSFC(31770112)for the financial support 

出 版 物：《BioDesign Research》 (生物设计研究（英文）)

年 卷 期：2022年第2022卷第1期

页      码：52-67页

摘      要：The majority of marine microbes remain uncultured,which hinders the identification and mining of CO_(2)-fixing genes,pathways,and chassis from the ***,we investigated CO_(2)-fixing microbes in seawater from the euphotic zone of the Yellow Sea of China by detecting and tracking their ^(13)C-bicarbonate(^(13)C-HCO_(3)^(-))intake via single-cell Raman spectra(SCRS)*** target cells were then isolated by Raman-activated Gravity-driven Encapsulation(RAGE),and their genomes were amplified and sequenced at one-cell *** single-cell metabolism,phenotype and genome are *** identified a notyet-cultured Pelagibacter spp.,which actively assimilates ^(13)C-HCO_(3)^(-),and also possesses most of the genes encoding enzymes of the Calvin-Benson cycle for CO_(2) fixation,a complete gene set for a rhodopsin-based light-harvesting system,and the full genes necessary for carotenoid *** four proteorhodopsin(PR)genes identified in the Pelagibacter *** confirmed by heterologous expression in *** results suggest that hitherto uncultured Pelagibacter *** light-powered metabolism to contribute to global carbon cycling.

主 题 词：metabolism Benson hinder 

学科分类：1002[医学-临床医学类] 100214[100214] 10[医学] 

D　O　I：10.34133/2022/9782712

馆 藏 号：203121498...