Enhancement of intranasalmucosal immunization of mucosal vaccines by ultrasonic treatment

作     者：Haowei Xu Yang Liao Mankovskaya Svetlana Deguang Yang Huaibin Wan Zonghua Liu Haowei Xu;Yang Liao;Mankovskaya Svetlana;Deguang Yang;Huaibin Wan;Zonghua Liu

作者机构：Department of Biomedical EngineeringJinan UniversityGuangzhou 510632China Department of Laboratory MedicineGeneral Hospital of Southern Theatre Command of PLAGuangzhou 510010China Institute of Physiology of NAS of BelarusMinskBelarus Department of CardiologyHeyuan Shenhe People’s HospitalThe Fifth Affiliated HospitalJinan UniversityHeyuan 517475China 

基　　金：supported by the National Key R&D Program of China(No.2018YFC0311103) 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第4期

页      码：405-422页

摘      要：The pathogens of most infectious diseases invade the host through mucosal sites,and immunization with mucosal vaccines is the best means of combating these infectious *** delivery and nasal delivery are the most common methods of mucosal ***,the delivery process is inefficient,and mucosal vaccination is ineffective because the vaccine formulation is easily and rapidly removed and has difficulty in crossing the mucosal *** this paper,we investigated whether the mucosal immune response could be enhanced by ultrasound facilitation of nasal mucosal delivery of vaccine *** this purpose,we used manganese dioxide(MnO2)as the vaccine carrier/adjuvant,coated with chitosan oligosaccharide(COS)to enhance mucosal adsorption,and further physically adsorbed model antigen ovalbumin(OVA)to construct a nanoparticulate vaccine formulation MnO2@COS@*** treatment was found to promote antigen delivery and recruitment of dendritic cells(DCs)and macrophages as well as T-cell infiltration in nasal mucosal tissues through nasal mucosal immunization *** ultrasound assistance,MnO2@COS@OVA particles promoted the maturation of DCs in vitro and in vivo and promoted the production of effector memory T cells in vivo and cytokine secretion by splenocytes in *** particular,ultrasound treatment significantly increased the levels of secretory IgA antibodies in the nasal mucosa and genital tract mucosa of experimental *** addition,the experimental data showed that the MnO2@COS@OVA particles had good biocompatibility and caused no significant damage to the nasal mucosal and vital organ *** data suggest that ultrasound treatment can promote the induction of efficient immune responses to mucosal vaccines and provide new ideas for the opening and clinical translation of mucosal vaccines.

主 题 词：Mucosal vaccine Ultrasound treatment MnO2 Nasal delivery 

学科分类：0831[工学-公安技术类] 1001[医学-基础医学] 100102[100102] 10[医学] 

核心收录：

D　O　I：10.1007/s42242-023-00231-9

馆 藏 号：203121733...