3D-printed engineered bacteria-laden gelatin/sodium alginate composite hydrogels for biological detection of ionizing radiation

作     者：Ziyuan Chen Jintao Shen Meng Wei Wenrui Yan Qiucheng Yan Zhangyu Li Yaqiong Chen Feng Zhang Lina Du Bochuan Yuan Yiguang Jin Ziyuan Chen;Jintao Shen;Meng Wei;Wenrui Yan;Qiucheng Yan;Zhangyu Li;Yaqiong Chen;Feng Zhang;Lina Du;Bochuan Yuan;Yiguang Jin

作者机构：Department of Pharmaceutical SciencesBeijing Institute of Radiation MedicineBeijing 100850China 

基　　金：supported by the Special Program for Capability Promotion 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第4期

页      码：439-450页

摘      要：Nuclear safety is a global growing concern,where ionizing radiation(IR)is a major injury factor resulting in serious damage to *** detection of IR is usually conducted with physical dosimeters;however,biological IR detection methods are ***,a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate was 3D-printed for the biological detection of *** strains of PrecA::egfp gene circuit-containing engineered Escherichia coli were constructed with IR-dependent fluorescence,and the DH5αstrain was finally selected due to its highest radiation response and *** bacteria were loaded in a series of gelatin/sodium alginate matrix hydrogels with different rheology,3D printability and bacterial applicability.A high-gelatin-content hydrogel containing 10%gelatin/1.25%sodium alginatewas *** optimal living composite hydrogelwas 3D-printedwith the special bioink,which reported significant green fluorescence underγ-ray *** living composite hydrogel provides a biological strategy for the detection of environmental ionizing radiation.

主 题 词：3D printing Alginate Engineered bacteria Gelatin Hydrogel Ionizing radiation 

学科分类：081702[081702] 08[工学] 0817[工学-轻工类] 0837[0837] 080501[080501] 0805[工学-能源动力学] 080502[080502] 0703[理学-化学类] 0702[理学-物理学类] 

核心收录：

D　O　I：10.1007/s42242-023-00238-2

馆 藏 号：203122024...