Highly specific characterization and discrimination of monosodium urate crystals in gouty arthritis based on aggregation-induced emission luminogens

作     者：Wenjuan Wang Guiquan Zhang Ziyi Chen Hanlin Xu Bohan Zhang Rong Hu Anjun Qin Yinghui Hua Wenjuan Wang;Guiquan Zhang;Ziyi Chen;Hanlin Xu;Bohan Zhang;Rong Hu;Anjun Qin;Yinghui Hua

作者机构：Department of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China State Key Laboratory of Luminescent Materials and DevicesGuangdong Provincial Key Laboratory of Luminescence from Molecular AggregatesSouth China University of TechnologyGuangzhou 510640China Center for Aggregation-Induced EmissionAlE InstituteSouth China University of TechnologyGuangzhou 510640China School of Chemistry and Chemical EngineeringUniversity of South ChinaHengyang 421001China State Key Laboratory of Surface PhysicsDepartment of PhysicsFudan UniversityShanghai 200433China 

基　　金：Thisworkwas supported by the Shanghai Science and Technology Committee(No.22dz1204700) the NationalKeyR&D Program of China(Nos.2020YFA0803800 and 2017YFE0132200) the National Natural Science Foundation of China(Nos.82072510,21907034,21788102,21525417,and 51620105009) the Natural Science Foundation of Guangdong Province(Nos.2019B030301003 and 2016A030312002) the Innovation and Technology Commission of Hong Kong(No.ITC-CNERC14S01) 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第6期

页      码：704-717页

摘      要：Existing technologies used to detect monosodium urate(MSU)crystals for gout diagnosis are not ideal due to their low sensitivity and complexity of *** purpose of this study was to explore whether aggregation-induced emission luminogens(AIEgens)can be used for highly specific imaging of MSU crystals to assist in the diagnosis of ***,we developed a series of luminogens(i.e.,tetraphenyl ethylene(TPE)-NH_(2),TPE-2NH_(2),TPE-4NH_(2),TPE-COOH,TPE-2COOH,TPE-4COOH,and TPE-Ketoalkyne),each of which was then evenly mixed with MSU ***,optimal fluorescence imaging of each of the luminogens was characterized by a confocal laser scanning microscope(CLSM).This approach was used for imaging standard samples of MSU,hydroxyapatite(HAP)crystals,and mixed samples with 1:1 mass ratio of MSU/*** also imaged samples from mouse models of acute gouty arthritis,HAP deposition disease,and comorbidities of ***,CLSM imaging results were compared with those of compensated polarized light microscopy,and we assessed the biosafety of TPE-Ketoalkyne in the RAW264.7 cell ***,CLSM time series and three-dimensional imaging were performed on MSU crystal samples from human gouty synovial fluid and *** a promising candidate for MSU crystal labeling,TPE-Ketoalkyne was found to detect MSU crystals accurately and rapidly in standard samples,animal samples,and human samples,and could precisely distinguish gout from HAP deposition *** work demonstrates that TPE-Ketoalkyne is suitable for highly specific and timely imaging of MSU crystals in gouty arthritis and may facilitate future research on MSU crystal-related diseases.

主 题 词：Gout Monosodium urate Hydroxyapatite TPE-Ketoalkyne Aggregation-induced emission Confocal laser scanning microscope imaging 

学科分类：08[工学] 080501[080501] 0805[工学-能源动力学] 080502[080502] 0803[工学-仪器类] 

核心收录：

D　O　I：10.1007/s42242-023-00252-4

馆 藏 号：203124370...