A novel integrated microfluidic chip for on-demand electrostatic droplet charging and sorting

作     者：Jinhui Yao Chunhua He Jianxin Wang Canfeng Yang Ye Jiang Zhiyong Liu Guanglan Liao Tielin Shi Jinhui Yao;Chunhua He;Jianxin Wang;Canfeng Yang;Ye Jiang;Zhiyong Liu;Guanglan Liao;Tielin Shi

作者机构：State Key Laboratory of Intelligent Manufacturing Equipment and TechnologyHuazhong University of Science and TechnologyWuhan 430074China 

基　　金：The authors acknowledge the financial support from the NationalNatural Science Foundation ofChina(No.52275562) the Technology Innovation Fund of Huazhong University of Science and Technology(No.2022JYCXJJ015) 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第1期

页      码：31-42页

摘      要：On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual ***,state-of-the-art microfluidic chips for droplet sorting still suffer from low sorting speeds,sample loss,and labor-intensive preparation ***,we demonstrate the development of a novel microfluidic chip that integrates droplet generation,on-demand electrostatic droplet charging,and high-throughput *** charging electrode is a copper wire buried above the nozzle of the microchannel,and the deflecting electrode is the phosphate buffered saline in the microchannel,which greatly simplifies the structure and fabrication process of the ***,this chip is capable of high-frequency droplet generation and sorting,with a frequency of 11.757 kHz in the drop *** chip completes the selective charging process via electrostatic induction during droplet ***-demand charged microdroplets can arbitrarilymove to specific exit channels in a three-dimensional(3D)-deflected electric field,which can be controlled according to user requirements,and the flux of droplet deflection is thereby significantly ***,a lossless modification strategy is presented to improve the accuracy of droplet deflection or harvest rate from 97.49% to 99.38% by monitoring the frequency of droplet generation in real time and feeding it back to the charging *** chip has great potential for quantitative processing and analysis of single cells for elucidating cell-to-cell variations.

主 题 词：Copper wire Droplet generation Droplet sorting Microfluidic chips On-demand charging 

学科分类：0831[工学-公安技术类] 080903[080903] 0809[工学-计算机类] 08[工学] 1010[医学-医学技术类] 0802[工学-机械学] 10[医学] 080201[080201] 

核心收录：

D　O　I：10.1007/s42242-023-00257-z

馆 藏 号：203124962...