Multidimensional Optimization of Saccharomyces cerevisiae for Carotenoid Overproduction

作     者：Jian Fan Yang Zhang Wenhao Li Zhizhen Li Danli Zhang Qiwen Mo Mingfeng Cao Jifeng Yuan Jian Fan;Yang Zhang;Wenhao Li;Zhizhen Li;Danli Zhang;Qiwen Mo;Mingfeng Cao;Jifeng Yuan

作者机构：State Key Laboratory of Cellular Stress BiologySchool of Life SciencesFaculty of Medicine and Life SciencesXiamen UniversityFujian 361102China Shenzhen Research Institute of Xiamen UniversityShenzhen 518057China College of Chemistry and Chemical EngineeringXiamen UniversityFujian 361005China Key Laboratory for Synthetic Biotechnology of Xiamen CityXiamen UniversityFujian 361005China 

基　　金：support from the National Natural Science Foundation of China(32270087) the Natural Science Foundation of Fujian Province of China(2020J05011) Guangdong Basic and Applied Basic Research Foundation(2021A1515110340) Xiamen University(0660X2510200) Daan Gene(20223160A0063),and ZhenSheng Biotech 

出 版 物：《BioDesign Research》 (生物设计研究（英文）)

年 卷 期：2024年第6卷第1期

页      码：20-27页

摘      要：Microbial synthesis of carotenoids is a highly desirable alternative to plant extraction and chemical *** this study,we investigated multidimensional strategies to improve the carotenoid synthesis in the industrial workhorse of Saccharomyces ***,we rewired the yeast central metabolism by optimizing non-oxidative glycolysis pathway for an improved acetyl-CoA ***,we restricted the consumption of farnesyl pyrophosphate(FPP)by the down-regulation of squalene synthase using the PEST ***,we further explored the human lipid binding/transfer protein saposin B(hSapB)-mediated metabolic sink for an enhanced storage of lipophilic ***,the copper-induced GAL expression system was engineered to function in the yeast-peptone-dextrose medium for an increased biomass *** combining the abovementioned strategies,the final engineered yeast produced 166.79±10.43 mg/Ⅰβ-carotene in shake flasks,which was nearly 5-fold improvement of the parental carotenoid-producing ***,we envision that multidimensional strategies reported here might be applicable to other hosts for the future industrial development of carotenoid synthesis from renewable feedstocks.

主 题 词：optimization dimension siona multi carotenoid cerevisiae overproduction saccharomyces 

学科分类：081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 070303[070303] 0703[理学-化学类] 

D　O　I：10.34133/bdr.0026

馆 藏 号：203125808...