Biofabrication of nanocomposite-based scaffolds containing human bone extracellularmatrix for the differentiation of skeletal stem and progenitor cells

作     者：Yang-Hee Kim Janos M.Kanczler Stuart Lanham Andrew Rawlings Marta Roldo Gianluca Tozzi Jonathan I.Dawson Gianluca Cidonio Richard O.C.Oreffo Yang-Hee Kim;Janos M.Kanczler;Stuart Lanham;Andrew Rawlings;Marta Roldo;Gianluca Tozzi;Jonathan I.Dawson;Gianluca Cidonio;Richard O.C.Oreffo

作者机构：Faculty of MedicineBone and Joint Research GroupCentre for Human DevelopmentStem Cells and RegenerationInstitute of Developmental SciencesUniversity of SouthamptonSouthampton SO166YDUK School of Pharmacy and Biomedical ScienceUniversity of PortsmouthPortsmouth PO12DTUK School of EngineeringFaculty of Engineering and ScienceUniversity of GreenwichGreenwich ME44TBUK Center for Life Nano-and Neuro-Science(CLN2S)Italian Institute of Technology00161 RomeItaly 

基　　金：supported by grants from the Biotechnology and Biological Sciences Research Council(Nos.BBSRC LO21071/and BB/L00609X/1) UK Regenerative Medicine Platform Hub Acellular Approaches for Therapeutic Delivery(No.MR/K026682/1) Acellular Hub,SMART Materials 3D Architecture(No.MR/R015651/1) the UK Regenerative Medicine Platform(No.MR/L012626/1 Southampton Imaging)to ROCO MRCAMED Regenerative Medicine and Stem Cell Research Initiative(No.MR/V00543X/1)to JID,ROCO and YHK GC acknowledges funding from AIRC Aldi Fellowship under grant agreement No.25412 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第2期

页      码：121-136页

摘      要：Autograft or metal implants are routinely used in skeletal ***,they fail to provide long-term clinical resolution,necessitating a functional biomimetic tissue engineering *** use of native human bone tissue for synthesizing a biomimeticmaterial inkfor three-dimensional(3D)bioprintingof skeletal tissueis anattractivestrategyfor ***,human bone extracellular matrix(bone-ECM)offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and differentiate along the osteogenic *** this study,we engineered a novel material ink(LAB)by blending human bone-ECM(B)with nanoclay(L,Laponite®)and alginate(A)polymers using extrusion-based *** inclusion of the nanofiller and polymeric material increased the rheology,printability,and drug retention properties and,critically,the preservation of HBMSCs viability upon *** composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)*** inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced vascularization and mineralization after only seven *** study demonstrates the synergistic combination of nanoclay with biomimetic materials(alginate and bone-ECM)to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.

主 题 词：Extracellular matrix Nanoclay Bone 3D bioprinting 

学科分类：0831[工学-公安技术类] 08[工学] 0836[0836] 

核心收录：

D　O　I：10.1007/s42242-023-00265-z

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