A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

作     者：I.A.O.Beeren G.Dos Santos P.J.Dijkstra C.Mota J.Bauer H.Ferreira Rui L.Reis N.Neves S.Camarero-Espinosa M.B.Baker L.Moroni I.A.O.Beeren;G.Dos Santos;P.J.Dijkstra;C.Mota;J.Bauer;H.Ferreira;Rui L.Reis;N.Neves;S.Camarero-Espinosa;M.B.Baker;L.Moroni

作者机构：Department of Complex Tissue RegenerationMERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research GroupI3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory4806-909 Braga/GuimarãesPortugal POLYMATUniversity of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUEBasque Foundation for Science48009 BilbaoSpain 

基　　金：the European Research Council starting grant “Cell Hybridge” for financial support under the Horizon2020 framework program (Grant#637308) the Province of Limburg for support and funding 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第3期

页      码：277-291页

摘      要：Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

主 题 词：Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human 

学科分类：08[工学] 080501[080501] 0805[工学-能源动力学] 080502[080502] 

核心收录：

D　O　I：10.1007/s42242-024-00286-2

馆 藏 号：203127899...