Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody

作     者：Gao-Qin Liu, Xue-Guang Zhang 

作者机构：Department of Ophthalmology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China Jiangsu Institute of Clinic Immunology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China 

基　　金：Supported by National Natural Science Foundation in China(NSFC No.30771978and No30972712) Qing-Lan Project of Education Bureau of Jiangsu Province Supported by Jiangsu Province's Key Provincial Talents Program,China(No.RC2011104) 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2012年第5卷第1期

页      码：7-12页

摘      要：AIM: To explore the effect of SDF-1α on the development of experimental corneal neovascularization (CRNV).METHODS: CRNV was induced by alkali injury in mice. The expression of SDF-1α and CXCR4 in burned corneas was examined by Flow Cytometry. Neutralizing anti-mouse SDF-1α antibody was locally administrated after alkali injury and the formation of CRNV 2 weeks after injury was assessed by Immunohistochemistry. The expression of VEGF and C-Kit in burned corneas was detected by ***: The number of CRNV peaks at 2 weeks after alkali injury. Compared to control group, SDF-1α neutralizing antibody treatment significantly decreased the number of CRNV. RT-PCR confirmed that SDF-1α neutralizing antibody treatment resulted in decreased intracorneal VEGF and C-Kit ***: SDF-1α neutralizing antibody treated mice exhibited impaired experimental CRNV through down regulated VEGF and C-Kit expression.

主 题 词：corneal neovascularization alkali injury chemokine 

学科分类：1002[医学-临床医学类] 100212[100212] 10[医学] 

核心收录：

D　O　I：10.3980/j.issn.2222-3959.2012.01.02

馆 藏 号：203131023...