Microfluidic thermotaxic selection of highlymotile sperm and in vitro fertilization

作     者：Sihan Chen Jiemin Chen Zihan Qin Jibo Wang Yuwen Wang Rong Liu Wen Zhao Ming Zhang Yuanzhen Zhang Mengcheng Luo Pu Chen 

作者机构：Tissue Engineering and Organ Manufacturing(TEOM)LabDepartment of Biomedical EngineeringTaiKang Medical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated DiseaseTaiKangMedical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Reproductive Medicine CenterZhongnan Hospital of Wuhan UniversityWuhan 430071China TaiKang Center for Life and Medical SciencesWuhan UniversityWuhan 430071China 

基　　金：supported by the Key Research and Development Project of Hubei Province China(No.2021BCA111) 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第5期

页      码：687-700页

摘      要：The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic *** adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to *** a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment *** this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is *** by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and *** study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm *** indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis *** thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation ***,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to *** microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.

主 题 词：Microfluidic device Thermotaxis Sperm sorting Assisted reproductive technology In vitro fertilization 

学科分类：1002[医学-临床医学类] 080103[080103] 100210[100210] 0802[工学-机械学] 0811[工学-水利类] 0801[工学-力学类] 10[医学] 

核心收录：

D　O　I：10.1007/s42242-024-00306-1

馆 藏 号：203131285...