山楂海棠嫩茎直接再生芽苗体系及种质离体保存

作     者：顾地周 高捍东 张庆增 王玉方 冯颖 

作者机构：通化师范学院生物系吉林通化134002 南京林业大学森林资源与环境学院江苏南京210037 农业部特种经济动植物及产品质量监督检验测试中心吉林吉林132109 

基　　金：吉林省科技厅资助项目(200705C05) 

出 版 物：《林业科学研究》 (Forest Research)

年 卷 期：2009年第22卷第6期

页      码：888-892页

摘      要：The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design *** results showed that tissue culture of *** required different kinds of culture medium in different ***+TDZ 2.45 mg·L-1+NAA 0.06 mg·L-1 for shoots regeneration immediately at base of tender stem,the rate of induction was 98.8%;1/4 SH+KT 0.35 mg·L-1+NAA 0.05 mg·L-1 for rooting,the rate of rooting was more than 97%;1/8 SH+ABA 2.05 mg·L-1+KT 0.50 mg·L-1 for germplasm preservation in vitro,the rate of dormancy was 95.5%.These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.

主 题 词：山楂海棠 植株再生 种质离体保存 均匀设计 

学科分类：09[农学] 0902[农学-自然保护与环境生态类] 090201[090201] 

核心收录：

D　O　I：10.3321/j.issn:1001-1498.2009.06.025

馆 藏 号：203139440...