A LFD-RAA-Based Rapid Detection Method for Pseudomonas syringae pv. tabaci

作     者：XIAO Yan-song LI Hong-guang LI Si-jun WU Wen-xin ZHOU Lu-su ZHONG Jie SU Jia-en YANG Zhi-juan 肖艳松;李宏光;李思军;吴文信;周陆苏;钟杰;苏家恩;杨志娟

作者机构：Chenzhou Tobacco CompanyHunan Provincial Tobacco CorporationChenzhou 423000PRC Guiyang Branch of Chenzhou Tobacco CompanyHunan Provincial Tobacco CorporationChenzhou 424400PRC College of Plant ProtectionHunan Agricultural UniversityChangsha 410128PRC Dali Tobacco CompanyYunnan Provincial Tobacco CorporationDali 671000PRC 

基　　金：湖南省郴州市烟草公司烟草农业科技创新项目(CZYC2022JS03,CZYC2023JS05) 云南省烟草公司大理州公司科技计划一般项目(DLYC2022003) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2024年第25卷第2期

页      码：38-43页

摘      要：This study aimed to achieve rapid detection of Pseudomonas syringae ***,the pathogen of tobacco wildfire *** specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target *** was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the ***,the detection performance of the established method was *** results showed that the LFDRAA method had high *** amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR ***,the LFD-RAA method could quickly detect *** *** from tobacco leaves,demonstrating field *** sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.

主 题 词：Tobacco wildfire disease Recombinase-aided amplification Detection 

学科分类：09[农学] 0904[农学-动物医学类] 090401[090401] 090402[090402] 

D　O　I：10.16175/j.cnki.1009-4229.2024.02.007

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