Ca_v3.3-mediated endochondral ossification in a three-dimensional bioprinted Gel MA hydrogel

作     者：Zhi Wang Xin Wang Yang Huang Junjun Yang Zu Wan Zhenlan Fu Xiaoyuan Gong Guangxing Chen Liu Yang Zhi Wang;Xin Wang;Yang Huang;Junjun Yang;Zu Wan;Zhenlan Fu;Xiaoyuan Gong;Guangxing Chen;Liu Yang

作者机构：Chongqing Key Laboratory of Precision Medicine in Joint SurgeryCenter for Joint SurgerySouthwest HospitalArmy Medical University(Third Military Medical University)Chongqing 400038China Department of Pharmacy and Laboratory MedicineArmy Medical University(Third Military Medical University)Chongqing 400038China Key Laboratory of Biorheological Science and TechnologyMinistry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044China 

基　　金：supported by the National Natural Science Foundation of China(No.31800784) the Chongqing Key Laboratory of Precision Medicine in Joint Surgery(No.425Z2138) the Chongqing Excellent Scientist Project(No.425Z2W21) the Chongqing Natural Science Foundation General Project(No.cstc2021jcyjmsxm X0135) the Chongqing Postdoctoral Research Project Special Fund(No.2021XM3033) 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第6期

页      码：983-999页

摘      要：The growth plate(GP)is a crucial tissue involved in skeleton development via endochondral ossification(EO).The bone organoid is a potential research model capable of simulating the physiological function,spatial structure,and intercellular communication of native ***,mimicking the EO process remains a key challenge for bone organoid *** simulate this orderly mineralization process,we designed an in vitro sh Ca_(v)3.3 ATDC5-loaded gelatin methacryloyl(Gel MA)hydrogel model and evaluated its bioprintability for future organoid *** this paper,we report the first demonstration that the T-type voltage-dependent calcium channel(T-VDCC)subtype Ca_(v)3.3 is dominantly expressed in chondrocytes and is negatively correlated with the hypertrophic differentiation of chondrocytes during the EO ***,Ca_(v)3.3 knockdown chondrocytes loaded with the Gel MA hydrogel successfully captured the EO process and provide a bioink capable of constructing layered and orderly mineralized GP organoids in the *** results of this study could therefore provide a potential target for regulating the EO process and a novel strategy for simulating it in bone organoids.

主 题 词：Bone organoid Endochondral ossification T-type voltage-dependent calcium channel(T-VDCC) Ca_(v)3.3 3D bioprinting 

学科分类：08[工学] 080501[080501] 0805[工学-能源动力学] 080502[080502] 

核心收录：

D　O　I：10.1007/s42242-024-00287-1

馆 藏 号：203155717...