Cloning and Sequence Analysis of Lactate Dehydrogenase C(LDH-C)Gene from Black-lipped Pika in Western Sichuan Plateau

作     者：贺庆华 徐亚欧 郑玉才 陈锋 张文磊 HE Qing-hua;XU Ya-ou;ZHEN Yu-cai;CHEN Feng;ZHANG Wen-lei

作者机构：西南民族大学生命科学与技术学院四川成都610041 

基　　金：Supported by State Ethnic Affairs Commission of P.R.C.(08XN04) Applicable and Fundamental Research Funds of SichuanProvince(2008JY0068) Academic Culture and TechnologyLeaders in Sichuan Province Foundation 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2009年第10卷第6期

页      码：63-66页

摘      要：[Objective] The aim was to lay a foundation for research of contraceptive rodenticide with LDH-C4 as target protein. [ Method] EST sequence of LDH-C gene from black-lipped pika was cloned by PCR with degenerate primers; then the full length open reading frame （ORF） and 3＇UTR sequence were cloned by RACE technique. [ Result] The full length cDNA was 1 498 bp containing an ORF of 996 bp and a 3＇UTR of 486 bp. The ORF encoded a polypeptide of 332 amino acids. The alignment of LDH-C gene ORF nucleotide sequences from different species showed that the gene was conserved even between large taxons. The phylogenic tree showed that black-lipped pika LDH-C was closer to prima- tes and artiodactyla than to rodents. [Conclusion] cDNA sequence of LDH-C gene from black-lipped pika was cloned successfully.

主 题 词：LDH-C gene RACE EST Sequence analysis 

学科分类：0710[理学-生物科学类] 07[理学] 0828[工学-建筑类] 08[工学] 09[农学] 071007[071007] 0901[农学-植物生产类] 0836[0836] 090102[090102] 

D　O　I：10.3969/j.issn.1009-4229-B.2009.06.020

馆 藏 号：203156102...