Wdr63 Deletion Aggravates Ulcerative Colitis Likely by Affecting Th17/Treg Balance and Gut Microbiota

作     者：ZHU Hao ZHU Meng-Yuan CAO Yang-Yang YANG Qiu-Bo FAN Zhi-Peng 朱豪;朱梦远;曹杨杨;杨秋波;范志朋

作者机构：Beijing Institute for Dental ResearchCapital Medical University School of StomatologyBeijing 100050China Beijing Laboratory of Oral HealthCapital Medical UniversityBeijing 100069China Research Unit of Tooth Development and RegenerationChinese Academy of Medical SciencesBeijing 100069China 

基　　金：国家自然科学基金(82130028) 国家重点研发计划(2022YFA1104401) 中国医学科学院医学创新基金(2019-I2M-5-031) 首都医科大学北京口腔医院创新研究项目(CXTD202204)资助 

出 版 物：《生物化学与生物物理进展》 (Progress In Biochemistry and Biophysics)

年 卷 期：2025年第52卷第1期

页      码：209-222页

摘      要：Objective Ulcerative colitis is a prevalent immunoinflammatory ***17/Treg cell imbalance and gut microbiota dysregulation are key factors in ulcerative colitis *** actin cytoskeleton contributes to regulating the proliferation,differentiation,and migration of Th17 and Treg ***63,a gene containing the WD repeat domain,participates in the structure and functional modulation of actin *** research indicates that WDR63 may serve as a regulator of cell migration and metastasis via actin polymerization *** article aims to explore the effect of Wdr63 deletion on Th17/Treg cells and ulcerative *** We constructed Wdr63-/-mice,induced colitis in mice using dextran sulfate sodium salt,collected colon tissue for histopathological staining,collected mesenteric lymph nodes for flow cytometry analysis,and collected healthy mouse feces for microbial diversity *** Compared with wild-type colitis mice,Wdr63-/-colitis mice had a more pronounced shortening of colonic tissue,higher scores on disease activity index and histological damage index,Treg cells decreased and Th17 cells increased in colonic tissue and mesenteric lymph nodes,a lower level of anti-inflammatory cytokine IL-10,and a higher level of pro-inflammatory cytokine *** addition,WDR63 has shown positive effects on maintaining intestinal microbiota *** maintains the balance of Bacteroidota and Firmicutes,promoting the formation of beneficial intestinal bacteria linked to immune *** Wdr63 deletion aggravates ulcerative colitis in mice,WDR63 inhibits colonic inflammation likely by regulating Th17/Treg balance and maintains intestinal microbiota homeostasis.

主 题 词：Wdr63 Th17/Treg ulcerative colitis inflammation immune microbiology 

学科分类：1001[医学-基础医学] 100102[100102] 10[医学] 

核心收录：

D　O　I：10.16476/j.pibb.2024.0148

馆 藏 号：203156848...