Analysis of Population Structure and Genetic Diversity of Authentic Medicinal Herb Dendrobium huoshanense Using ESTSSR Markers

作     者：WANG Hui SHI Lingling ZHOU Jue ZHOU Yuxuan 王晖;时玲玲;周珏;周雨璇

作者机构：College of Life SciencesAnhui Normal UniversityWuhu 241000AnhuiChina Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education InstitutesWuhu 241000AnhuiChina Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological ResourcesWuhu 241000AnhuiChina Department of PathologyWannan Medical CollegeWuhu 241002AnhuiChina 

基　　金：安徽省教育厅自然科学基金平台重点项目(KJ2020A0086) 

出 版 物：《生命科学研究》 (Life Science Research)

年 卷 期：2024年第28卷第6期

页      码：572-582,600页

摘      要：Understanding the intra-population genetic diversity and population genetic structure is beneficial for plant protection and *** huoshanense is an endangered authentic medicinal *** analyze the population differentiation and genetic diversity in ***,simple sequence repeat(SSR)markers were obtained by high-throughput transcriptome sequencing,and 10 expressed sequence tag(EST)-SSRs were studied in 182 Dendrobium individual samples from 15 populations distributed in Huoshan County,southwest Anhui *** deep sequencing of *** transcriptome,a total of 58202 unigenes were obtained,and 7781 SSRs with 1~6 base repeats were *** them,dinucleotide SSRs were the dominant repeating motifs(3119,40.085%),and the most common was AG/CT(2666,54.01%).Ten SSR primers were screened out from the designed 120 primer pairs for further polymorphism *** results showed that 75 alleles were detected,and the number of alleles at each locus ranged from 4 to 11,with an average of *** mean expected heterozygosity(H_(e)),observed heterozygosity(H_(o))and polymorphism information content(C_(pi))of the 10 SSR loci were 0.646,0.574,and 0.607,*** populations exhibited a high level of genetic diversity(H_(o)=0.591,H_(e)=0.557)and a moderate level of differentiation(F_(ST)=0.10069),and the genetic variation mainly existed within *** results of Structure and neighbour-joining(NJ)analysis based on 10 polymorphic primers showed that *** populations were clustered into 2 groups,and the populations KS,HSD,and JXZ had rich genetic diversity and multiple private alleles,suggesting that these populations could be used for genetic improvement of the *** study revealed frequency distribution of *** SSR loci,and successfully developed microsatellite genetic markers,which are expected to expand the repertoire of DNA markers for genetic study of ***.

主 题 词：Dendrobium huoshanense microsatellite expressed sequence tag-simple sequence repeat(ESTSSR) genetic diversity genetic structure 

学科分类：1008[医学-中药学类] 07[理学] 0713[0713] 10[医学] 

D　O　I：10.16605/j.cnki.1007-7847.2023.11.0216

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