Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails

作     者：Yue-jin PU Yan WU Xiao-juan XU Jin-ping DU Yan-zhang GONG 

作者机构：Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of the Ministry of EducationHuazhong Agricultural University Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding Hubei Academy of Agriculture Sciences 

基　　金：supported by the Open Project of Hubei Key Laboratory of Animal Embryo and Molecular Breeding(No.2015ZD146) China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2016年第17卷第8期

页      码：591-596页

摘      要：The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vas- oactive intestinal peptide receptor-1 （VIPR-1） was selected as the candidate gene for identifying traits of egg produc- tion. A single nucleotide polymorphism （SNP） detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Two mutations （G373T, A313G） were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were sig- nificantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium （LD） analysis in- dicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the hlh2 （GGGT） diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.

主 题 词：Laying quail Haplotypes VIPR-1 gene Egg production traits 

学科分类：0905[农学-林学类] 09[农学] 

核心收录：

D　O　I：10.1631/jzus.B1500199

馆 藏 号：203171621...