Cloning and Sequence Analysis of CHS Gene Fragment from Acer truncatum

作     者：马立敏 丰震 齐新玲 Limin MA;Zhen FENG;Xinling QI

作者机构：山东农业大学林学院山东泰安271018 

基　　金：Supported by Agricultural Improved Variety Project of Shandong Province(LKZNo.96) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2016年第17卷第7期

页      码：1658-1662,1678页

摘      要：[Objective] This study aimed to clone and analyze the sequence of CHS gene from Acer truncatum leaves. [Method] Using A. truncatum cultivars No.1-6 as experimental materials, total RNA was extracted from A. truncatum leaves with the modified CTAB method. CHS gene sequences were downloaded from the NCBI and aligned by BLAST. Degenerate primers were designed by DNAMAN and Primer- premier5 to amplify the target band. CHS gene fragment was amplified by RT-PCR and ligated to pMD18-T vector. The identified positive colonies were sequenced. [Result] A 1 365 bp fragment was amplified. Sequence analysis suggested that the obtained fragment encoded 365 amino acids and shared above 90% homology to nucleotide sequence of CHS gene from A. palmatum and A. [Conclusion] In this study, CHS gene was successfully cloned from A. truncatum for the first time, which laid the foundation for efficient utilization of CHS gene.

主 题 词：Acer truncatum Chalcone synthase Cloning Sequence analysis RT- PCR 

学科分类：0907[农学-草药学] 08[工学] 0829[工学-安全科学与工程类] 09[农学] 

D　O　I：10.16175/j.cnki.1009-4229.2016.07.029

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