Isolation of Rice EPSP Synthase cDNA and Its Sequence Analysis and Copy Number Determination

作     者：徐军望 魏晓丽 李旭刚 陈蕾 冯德江 朱祯 

作者机构：中国科学院遗传和发育生物学研究所北京100101 

基　　金：国家高科技计划基金 ( 86 3) ( 2 0 0 1AA2 2 2 2 5 1,2 0 0 1AA2 12 0 41) 国家自然科学基金 ( 395 80 0 12,39880 0 2 3) 国家转基因植物研究与产业化开发专项基金 Rockefefeller基金(RF970 0 1# 5 81 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2002年第44卷第2期

页      码：188-192页

摘      要：In order to isolate the total cDNA of rice (Oryza sativa L.) epsps gene, RT-PCR was carried out with template of rice first-strand cDNA and primers designed according to rice EPSP synthase genomic sequence obtained in previous study. A 1 585-bp cDNA fragment was amplified and cloned. The 1 585-bp cDNA contains an open reading frame (ORF) comprising of 1 533 nucleotides (nt) which encodes a 511 residue polypepetides, including 67 amino acids chloroplast transit peptide and 444 amino acids EPSP synthase mature peptide. A comparison between the EPSP synthase of different sources indicates that the mature peptide shows more than 51% identity except for the fungi EPSP synthase and the transit peptide shows considerably less sequence conservation. The copy number of rice epsps gene is estimated to be one copy per haploid rice genome using southern blot. RT-PCR indicated that rice epsps gene is expressed in rice leaves, endosperms and roots and has the highest expression level in leaves.

主 题 词：rice EPSP synthase cDNA sequence sequence analysis copy numbers expression 

学科分类：09[农学] 0901[农学-植物生产类] 

核心收录：

D　O　I：10.3321/j.issn:1672-9072.2002.02.012

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