Bioassay-Guided Extraction of Crude Fucose-Containing Sulphated Polysaccharides from Sargassum fusiforme with Response Surface Methodology

作     者：FU Zhifei LI Haihua LIU Hongbing HU Shuman LI Yueying WANG Mengxue GUAN Huashi 

作者机构：Key Laboratory of Marine Drugs of Chinese Ministry of Education School of Medicine and PharmacyOcean University of China 

基　　金：supported by the National High Technology Research and Development Program of China (863 Program) (2013AA093003) 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2016年第15卷第3期

页      码：533-540页

摘      要：The response surface methodology(RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides(c FCSP) from Sargassum fusiforme. The central composite design(CCD) was used with four variables, five levels, and four responses. The four variables were p H value of hydrochloric acid solution, extraction temperature(℃), ratio of liquid to raw material(m L g^(-1)), and extraction time(h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of c FCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the p H value of hydrochloric acid solution was 3.50; the extraction temperature was 100℃; the ratio of liquid to raw material was 15.00 m L g^(-1) and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, c FCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.

主 题 词：crude fucose-containing sulphated polysaccharides Sargassum fusiforme response surface methodology immuno-modulatory 

学科分类：0710[理学-生物科学类] 071001[071001] 0908[0908] 07[理学] 0707[理学-海洋科学类] 0815[工学-矿业类] 0824[工学-林业工程类] 

核心收录：

D　O　I：10.1007/s11802-016-2897-9

馆 藏 号：203182192...