Phosphomonoesterase Activities,Kinetics and Thermodynamics in a Paddy Soil After Receiving Swine Manure for Six Years

作     者：LI Liang LIANG Xin-Qiang LI Hua JI Yuan-Jing LIU Jin YE Yu-Shi TIAN Guang-Ming CHEN Ying-Xu LUO Yong-Ming 

作者机构：Institute of Environmental Science and TechnologyCollege of Environmental and Resources SciencesZhejiang University Zhejiang Academy of Agricultural Sciences Key Laboratory for Water Pollution Control and Environmental Safety Yantai Institute of Coastal Zone ResearchChinese Academy of Sciences 

基　　金：supported by the National Natural Science Foundation of China(Nos.21077088 41271314and 51008107) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2015年第25卷第2期

页      码：294-306页

摘      要：Soil phosphomonoesterase plays a critical role in controlling phosphorus(P) cycling for crop nutrition,especially in P-deficient soils.A 6-year field experiment was conducted to evaluate soil phosphomonoesterase activities,kinetics and thermodynamics during rice growth stages after consistent swine manure application,to understand the impacts of swine manure amendment rates on soil chemical and enzymatic properties,and to investigate the correlations between soil enzymatic and chemical *** experiment was set out in a randomized complete block design with three replicates and five treatments including three swine manure rates(26,39,and 52 kg P ha^(-1),representing low,middle,and high application rates,respectively) and two controls(no-fertilizer and superphosphate at 26 kg P ha^(-1)).The results indicated that the grain yield and soil chemical properties were significantly improved with the application of P-based swine manure from 0 to 39 kg P ha^(-1);however,the differences between the 39(M_(39)) and 52 kg P ha^(-1) treatments(M_(52)) were not *** enzymatic property analysis indicated that acid phosphomonoesterase was the predominant phosphomonoesterase in the tested *** M_(39) and M_(52) treatments had relatively high initial velocity(V_0),maximal velocity(V_(max)),and activation grade(lgN_a) but low Michaelis constant(K_m),temperature coefficient(Q_(10)),activation energy(E_a),and activation enthalpy(ΔH),implying that the M_(39) and M_(52) treatments could stimulate the enzyme-catalyzed reactions more easily than all other *** correlation analysis showed that the distribution of soil phosphomonoesterase activities mainly followed the distributions of total C and total *** on these results,39 kg P ha^(-1) could be recommended as the most appropriate rate of swine manure amendment.

主 题 词：activation energy activation enthalpy enzyme-catalyzed reaction maximal velocity Michaelis constant 

学科分类：07[理学] 0713[0713] 

核心收录：

D　O　I：10.1016/S1002-0160(15)60014-5

馆 藏 号：203188231...