Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering

作     者：Chun-mao HAN Li-ping ZHANG Jin-zhang SUN Hai-fei SHI Jie ZHOU Chang-you GAO 

作者机构：Department of Burn the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027 China 

基　　金：Project supported by the National Basic Research Program (973) of China (No. 2005CB623902-1) the Science Research Foundation of the Ministry of Health of China (No. WKJ2006-2-2007) 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2010年第11卷第7期

页      码：524-530页

摘      要：To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.

主 题 词：关键词骨胶原 Chitosan 血纤维蛋白胶水 支架 设计织物的皮肤 

学科分类：0710[理学-生物科学类] 1007[医学-药学类] 1004[医学-公共卫生预防医学类] 1002[医学-临床医学类] 1001[医学-基础医学] 0905[农学-林学类] 100210[100210] 0906[农学-水产类] 10[医学] 

核心收录：

D　O　I：10.1631/jzus.B0900400

馆 藏 号：203195086...