A Graphene Oxide-based Immuno-biosensor for Vibrio parahaemolyticus Detection

作     者：王淑娴 王晓璐 曲梁静 盖春蕾 李乐 叶海斌 李天保 Shuxian WANG;Xiaolu WANG;Liangjing QU;Chunlei GAI;Le LI;Haibin YE;Tianbao LI

作者机构：山东省海洋生物研究院病害与渔药研究中心山东青岛266104 

基　　金：Supported by Shandong Scientific and Technological Development Program(2014GHY115024) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2017年第18卷第6期

页      码：1042-1045页

摘      要：Vibrio parahaemolyticus is the leading causal agent of human acute gas- troenteritis. Real-time accurate detection means is the key to prevention and control of its spread. This study provided a novel detection strategy for realizing rapid and specific determination of V. parahaemolyticus by labeling its monoclonal antibody （Ab） with quantum dots （QDs）. The results showed that the fluorescence of these QDs-Ab bioconjugates was quenched by graphene oxide （GO） to produce a bacteri- um capture probe. And the optimal quenched concentration of GO was 60 ng/ml. When the bacterium capture probe was exposed to the target, green color fluores- cence was turned on by releasing the QDs-Ab due to the antibody antigen combi- nation. The detection limit of V. parahaemolyticus was 104 CFU/ml based on 3 times signal-to-noise ratio. The specificity of the FRET sensor towards V. para- haemolyticus was examined by comparing with controls such as V. splendidus, V. alginolyticus, Edwardsiella tarda and Aeromonas hydrophila with the same condition. The controls couldn＇t cause obvious fluorescence alteration, while the target resulted in significant fluorescence enhancement. This strategy could be further used as a universal method for any bacterial determination by changing the conjugated antibod- ies in early disease diagnosis. Therefore, the sensor has good potential to expand its application to the early diagnosis and determination of bacteria.

主 题 词：Vibrio parahaemolyticus Graphene oxide Quantum dots FRET Im- muno-biosensor 

学科分类：0906[农学-水产类] 09[农学] 

D　O　I：10.16175/j.cnki.1009-4229.2017.06.019

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