IP_3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization

作     者：李洋洋 于涌鲲 万善霞 赵福宽 孙清鹏 于同泉 潘金豹 

作者机构：北京农学院生物技术学院北京102206 北京农学院植物科学技术学院北京102206 

基　　金：Supported by National Natural Science Foundation of China(30700428,30911130166) Natural Science Foundation of Beijing Municipality(5072009) The New Star Plan of Science and Technology in Beijing Municipality(2006B26) 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2011年第12卷第9期

页      码：1265-1269页

摘      要：[Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway.[Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2＋ which was pretreated with heparin on jasmonic acid（JA）-induced.[Results] When *** leaf cells were pretreated with 10,50 or 100 ng/ml heparin,intercellular free Ca2＋ fluorescence intensity was reduced in comparison with negative *** the heparin-pretreated *** leaf cells were stimulated with 100 μmol/L JA,intercellular Ca2＋ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control.[Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2＋ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.

主 题 词：Arabidopsis thaliana Ca2+ Heparin Jasmonic acid 

学科分类：0710[理学-生物科学类] 071001[071001] 07[理学] 

D　O　I：10.3969/j.issn.1009-4229-B.2011.09.006

馆 藏 号：203248384...