Advances in engineering methylotrophic yeast for biosynthesis of valuable chemicals from methanol
作者机构：Division of Biotechnology Dalian Institute of Chemical PhysicsChinese Academic Sciences
基 金：funded by the Young Investigator Grant from Dalian Institute of Chemicals Physics Chinese Academy of Sciences(to Y.J.Zhou)
出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)
年 卷 期：2018年第29卷第5期
页 码：681-686页
摘 要：Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, methylotrophic yeasts such as Pichia pastoris have been explored as a cell factory forproduction of proteins and high-value chemicals. Methanol utilization pathway （MUT） is highlyregulated for efficient methanol utilization, and the downstream pathways need extensively constructedand optimized toward target metabolite biosynthesis. Here, we present an overview of methanolmetabolism and regulation in methylotrophic yeasts, among which we focus on the regulation of keygenes involved in methanol metabolism. Besides, the recent progresses in construction and optimizationof downstream biosynthetic pathways for production of high value chemicals, such as polyketides, fattyacids and isoprenoids, are further summarized. Finally, we discuss the current challenges and feasiblestrategies toward constructing efficient methylotrophic cell factories may promote wide applications inthe future.
主 题 词：Microbial cell factory Methylotrophic yeast P. pastoris Methanol metabolism Biosynthetic pathways Metabolic engineering
学科分类：081702[081702] 08[工学] 0817[工学-轻工类]
核心收录：
D O I：10.1016/j.cclet.2017.11.015
馆 藏 号：203284993...
