Nontoxic engineered virus nanofibers as an efficient agent for the prevention and detection of fungal infection

作     者：Yicun Wang Hongxi Shi Shuai Dong Yan Li Meng Wang Yanyan Huai Xintong Zhang Xi Chen Chuanbin Mao Xiang Gao Li Wang 

作者机构：Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology Northeast Normal University Changchun 130024 China Jilin Provincial Key Laboratory on Molecular and Chemical Genetic The Second Hospital of Jilin University Changchun 130024 China Department of Respiratory Medicine Harbin Medical University Cancer Hospital Harbin 150081 China School of Materials Science and Engineering Zhejiang University Hangzhou 310027 China Department of Chemistry & Biochemistry Stephenson Life Science Research Center University of Oklahoma Norman 73019-5300 USA 

基　　金：This work was supported by the National Natural Science Foundation of China (No. 81373231)  the Department of Science and Technology of Jilin Province  China (No. 20160101216JC)  the Programme for Introducing Talents to Universities (No. B07017)  and Zhejiang Provincial Natural Science Foundation of China (No. LZ16E030001). The funders had no role in study design  data collection and analysis  decision to publish  or preparation of the manuscript 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2018年第11卷第4期

页      码：2248-2255页

摘      要：Candida albicans （C. albicans） infection has a high mortality rate in immunocompromised patients. Owing to the inefficiency of the current diagnostic system and the absence of licensed vaccines against candidiasis, the prevention of C. albicans infection remains a challenge. C. albicans infection can be evaluated and prevented by the anti-secreted aspartyl proteinase 2 antibody （anti-Sap2 IgG） and Hsp90 antibody （anti-Hsp90 IgG）. In this study, to explore a new agent for the improvement of the diagnosis and the prevention of C. albicans infection, an engineered fd bacteriophage, which is considered a human-safe virus nanofiber, was designed and prepared with two epitopes that could induce and capture anti-Sap2 IgG and anti-Hsp90 IgG. The dual-display phage was employed as a novel capture probe to develop a new enzyme-linked immunosorbent assay （ELISA） method, which significantly improved the detection rate compared with those of the ELISA in which recombinant protein Sap2 was used as coating antigen to capture the spedfic antibodies （rSap2-ELISA） and the ELISA in which recombinant protein Hsp90 was used as coating antigen to capture the specific antibodies （rHsp90-ELISA）. In addition, the nanofibers acted as a potential vaccine to immunize mice, as well as recombinant proteins, more efficiently mediated humoral and cellular immune responses, decreased levels of C. albicans colonization, and increased the survival rates in C. albicans-infected mice. Therefore, the phage dual-display nanofiber has been shown to be a powerful bifunctional agent for protection against and sensitive detection of clinical infections, which has the potential to be widely used in the life sciences, clinical medicine, and environmental sciences.

主 题 词：phage nanofiber Candida albicans diagnosis vaccine 

学科分类：090603[090603] 120202[120202] 12[管理学] 1202[管理学-工商管理类] 09[农学] 0906[农学-水产类] 

核心收录：

D　O　I：10.1007/s12274-017-1845-3

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