BODIPY-derived ratiometric fluorescent sensors: pH-regulated aggregation-induced emission and imaging application in cellular acidification triggered by crystalline silica exposure

作     者：Yang Bai Danyang Liu Zhong Han Yuncong Chen Zhongyan Chen Yang Jiao Weijiang He Zijian Guo 

作者机构：State Key Laboratory of Coordination Chemistry Coordination Chemistry Institute School of Chemistry and Chemical EngineeringNanjing University 

基　　金：supported by the National Basic Research Program of China (2015CB856300) the National Natural Science Foundation of China (21571099, 21731004) the Natural Science Foundation of Jiangsu Province (BK20150054) 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2018年第61卷第11期

页      码：1413-1422页

摘      要：Modification of classic fluorophore to possess the emission transitions between aggregation-induced emission(AIE)and intrinsic emission offers reliable approach to the design of ratiometric fluorescent *** this study,a proton acceptor benzimidazole was integrated with BODIPY to form three compounds,BBI-1/2/3,which demonstrated the AIE(~595 nm,I_(agg))in neutral aqueous medium and intrinsic BODIPYemission(~510 nm,I_(int))in acidic *** the three showed the ratiometric pH sensing behavior in a dual excitation/dual emission mode,yet BBI-3 displayed still the dual emission ratiometric pH sensing *** pH-dependent emission ratio I_(int)/I_(agg)of the three were fully reversible,and no interference was observed from normal abundant chemical species in live *** different p K_a(BBI-1,p K_a4.4;BBI-2,p K_a2.7;BBI-3,p K_a3.6)suggested that the substituents on benzimidazole moiety were essential to govern their functioning pH *** ratiometric imaging of BBI-1 in A549 cells provided an effective intracellular pH(pH_i)calibration formula corresponding to emission ratio of I_(int)/I_(agg).Ratiometric pH_iimaging in A549 cells upon small particle exposure confirmed the particle-induced cellular acidification with this *** particle size and the chemical nature of the particle contribute to the observed acidification *** synchronization of lysosome disruption to cellular acidification in A549 cells upon crystalline silica exposure was directly observed for the first time with BBI-1,showing the potential application of BBI-1 in the study of silicosis and other related *** study demonstrated that endowing fluorophore with AIE/intrinsic emission transition could be a promising strategy for ratiometric sensor design.

主 题 词：传感器设计 细胞内 荧光灯 排放 pH 发酸 水晶 成像 

学科分类：081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 080202[080202] 070302[070302] 0703[理学-化学类] 0802[工学-机械学] 

核心收录：

D　O　I：10.1007/s11426-018-9284-4

馆 藏 号：203308122...