Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone

作     者：Yu Dong Shen Yu Wang Shi Wei Zhang Zhi LiXiao Yuan Ming Sun Xian Zhang Bu Lian Quan Gu 

作者机构：Food Quality and Safety Key Laboratory of Department of Education of Guangdong Province/Institute of Food Quality and Safety South China Agricultural University Guangzhou 510642 China School of Pharmaceutical Sciences Sun Yat-Sen University Guangzhou 510080 China 

基　　金：Headmaster's Science Fund of High Schools, (5100-k06133) Public Bidding Items Fund for a Great Breakthrough, (2006A25005002) Natural Science Foundation of Guangdong Province, (06300421, 06300423) 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2007年第18卷第12期

页      码：1490-1492页

摘      要：A good strategy was brought forward for designing efficient haptens and complete antigens for 3-amino-2-oxazolidinone （AOZ）. Haptens designed newly were achieved facilely in good yield by using LiCI-N（Et）3 as new catalysis system, the structure of which was elucidated by spectroscopy analysis, such as NMR and MS. Novel antigens for AOZ were prepared successfully by convenient active ester method. The ratios of haptens 3 and 4 to carrier proteins were proven respectively as 41 ： 1 （5a）, 39：1 （6a）, 11：1 （5b） and 9：1 （6b） by trinitrobenzene sulfonic acid （TNBS） method. The results of indirect competitive ELISA （ic-ELISA） of antiserums indicated that the haptens with a short unsaturated side chain can evoke specific immune response effectively.

主 题 词：Furazolidone AOZ Antigen Hapten Immunoassay 

学科分类：1007[医学-药学类] 100701[100701] 10[医学] 

核心收录：

D　O　I：10.1016/j.cclet.2007.10.030

馆 藏 号：203363268...