Biomechanical researches on tissue engineering bone constructed by deproteinated bone

作     者：简月奎 田晓滨 李起鸿 李波 彭智 赵伟峰 王远政 杨震 

作者机构：Department of Orthopaedics Guizhou Provincial People's Hospital Guiyang 550002 China Center for Joint Surgery Southwest Hospital Third Military Medical University Chongqing 400038 China 

基　　金：This study was supported by the Science and Technology Foundation of Guizhou Province(No.20082303 200898 20093019) 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2010年第13卷第1期

页      码：32-36页

摘      要：Objective： To study biomechanical changes of newly formed bones 24 weeks after repairing large defects of long bones of goats using heterogeneous deproteinated bone （DPB） prepared by modified methods as an engineering scaffold. Methods： According to a fully randomized design, 18 goats were evenly divided into three groups： normal bone control group （Group A）, autologous bone group （Group B） and experimental group （Group C）. Each goat in Groups B and C were subjected to the periosteum and bone defect at middle-lower part of the right tibia （20% of the whole tibia in length）, followed by autologous bone or DPB plus autolognus MSCs ＋ rhBMP2 implantation, respectively and semi- ring slot fixation; while goats in Group A did not perform osteotomy. At 24 weeks after surgery, biomechanical tests were carried out on the tibias. Results： At 24 weeks after surgery, the results of anticompression test on tibias in three groups were recorded by a functional recorder presented as linear pressure-deformation curve. The shapes of the curves and their change tendency were similar among three groups. The ultimate pressure values were 10.74 MPa±1.23 MPa, 10. 11 MPa±1.35 MPa and 10.22 MPa±1.32 MPa and fracture compression rates were 26.82%±0.87%, 27.17%±0.75% and 28.22%±1.12% in Groups A, B and C, respectively. Comparisons of anti-compression ultimate pressures and fracture compression rates among three groups demonstrated no significant difference （PAB=0.415, PBC=0.494）. Three-point antibend test on tibias was recorded as load-deformation curves, and the shapes of the curves and their change tendency were similar among three groups. The ultimate pressure values of the anti-bend test were 481.52 N±12.45 N, 478.34 N±14.68 N and 475.62 N±13.41 N and the fracture bend rates were 2.62 mm±0.12 mm, 2.61 mm±0.15 mm and 2.81 mm±0.13 mm in Groups A, B and C, respectively. There was no significant difference between groups （PAB=0.7, PBc=0.448）. The ultimate anti-torsion torque values were 6.5

主 题 词：Biomechanics Tissue engineering Deproteinated bone 

学科分类：1002[医学-临床医学类] 100210[100210] 10[医学] 

D　O　I：10.3760/cma.j.issn.1008-1275.2010.01.006

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