Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology

作     者：Ling-Jia ZHAO Bo-Hou XIA Li-Mei LIN Su-Hui XIONG Jie TANG Duan-Fang LIAO 赵灵佳;夏伯侯;林丽美;熊苏慧;唐洁;廖端芳

作者机构：College of Pharmacy Hunan University of Chinese Medicine Changsha Hunan 410208 China Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province Hunan University of Chinese Medicine Changsha Hunan 410208 China Key laboratory for quality of bulk herbs for Hunan Province Hunan University of Chinese Medicine Changsha Hunan410208 China 

基　　金：funding support from the National Natural Science Foundation of China (No. 81503041) Project Foundation of Changsha Science and Technology Bureau (No. kq1701073) 

出 版 物：《Digital Chinese Medicine》 (数字中医药（英文）)

年 卷 期：2018年第1卷第3期

页      码：239-246页

摘      要：Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae *** Response surface methodology(RSM),combining Plackett–Burman design(PBD),steepest ascent method,and Box–Behnken design(BBD),was *** significant factors contributing to polysaccharide production were determined by *** path of steepest ascent method was performed to rapidly reach the neighborhood of the optimum *** and RSM were applied to further investigate the mutual interaction between the variables and to define the optimal *** The significant factors contributing to polysaccharide production were enzyme concentration,extraction time,and liquid-to-solid *** optimal conditions for maximal TFP were:enzyme concentration of2.8%,extraction time of41min,and liquid-to-solid ratio of27mL/*** experimental yield of3.08%was in good agreement with the model-predicted yield of3.1%under the optimized *** This study was successfully applied to optimize the extraction conditions of Turpiniae Folium polysaccharide,which can contribute to its further production and application.

主 题 词：Optimization Polysaccharides Turpinia argute Turpiniae Folium Response surface methodology 

学科分类：1008[医学-中药学类] 1006[医学-中西医结合类] 100602[100602] 10[医学] 

D　O　I：10.1016/S2589-3777(19)30031-X

馆 藏 号：203394130...