Molecular Design and Immunogenicity of a Multiple-epitope FMDV Antigen and DNA Vaccination

作     者：MA Ming-xiao JIN Ning-yi LIU Hui-juan ZHENG Mini YIN Ge-fen TIAN Ming-yao JIN Ming-lan LU Hui-jun SHEN Guo-shun ZHU Guang-ze JIN Hong-tao JIN Kuo-shi ZHANG Ying-jiu 

作者机构：Genetic Engineering Laboratory Academy of Military Medical Seienees Changehun 130062 P. R. China College of Animal Seienee and Veterinary Medieine Liaoning Medieal University Jinzhou 121001 P. R. China College of Animal Seienee and Veterinary Medieine Yunnan Agrieultural University Kunming 650201 P R. China Key Laboratory for Moleeular Enzymology and Engineering of Ministry of Edueation Jilin University Changehun 130021 P. R. China 

基　　金：National High Technology Research and Development Program of China(No.2006AA10A204) Educa-tional Department of Liaoning Province (No.20060527) 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2008年第24卷第1期

页      码：69-74页

摘      要：This article reports the design and construction of a multiple-epitope foot and mouth disease virus （FMDV） antigen, designated as OAAT. This recombinant antigen consists of the structural protein VP1 genes from serotypes A and O FMDV, five major VP1 immunodominant epitopes from two genotypes of Asial serotype, and three Th2 epitopes originating from the nonstructural protein, three ABC gene and structural protein VP4 gene. Expressions of target gene from these plasmids in HeLa cells were verified by Western-blot. BALB/c mice were immunized intramuscularly with the DNA vaccines thrice every two weeks. We found that pA could induce simultaneously specific antibodies against serotypes A, Asial, and O FMDV. Compared to those of the controls, the spots of FMDV-specific IFN-7 and cytotoxic activity from mice immunized with pA were significantly increased, pA provided full protection in 2/4 guinea pigs from challenge with FMDV O/NY00 and Asial/YNBS/58, respectively. The results show that although pA did not give full protection in 100% immunized guinea pigs from challenge with type O and Asial FMDV, respectively, OAAT may be potential immunogen against FMDV and pA may be potential DNA vaccines against FMDV.

主 题 词：Multiple-epitope Foot and mouth disease virus DNA vaccine 

学科分类：0710[理学-生物科学类] 071010[071010] 081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 070303[070303] 0703[理学-化学类] 

核心收录：

D　O　I：10.1016/S1005-9040(08)60015-X

馆 藏 号：203409580...