Design,synthesis and evaluation of spermine-based pH-sensitive amphiphilic gene delivery systems: Multifunctional non-viral gene carriers

作     者：XU RongZuo LU Zheng-Rong 

作者机构：Departments of Pharmaceutics and Pharmaceutical Chemistry University of Utah Salt Lake City Utah 84108 USA Department of Biomedical Engineering Case Western Reserve University Cleveland OH 44106 USA 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2011年第54卷第2期

页      码：359-368页

摘      要：Design and development of efficient non-viral gene delivery systems is critical to overcome various barriers for effective intracellular gene *** new spermine-based protonatable surfactants were designed,synthesized and evaluated as non-viral pH-sensitive gene *** carriers formed stable complexes with plasmid DNA at an N/P ratio as low as *** sizes of the carrier/pDNA nanoparticles (N/P = 12) were in the range of 90–130 nm,smaller than that of Lipofectamine2000/pDNA *** pDNA nanoparticles of the carriers exhibited substantially increased hemolysis when pH decreased from 7.4 to *** DNA nanoparticles had low cytotocixity,similar to that of Lipofectamine-2000/pDNA *** pH-sensitive gene carriers with no helper lipids resulted in much higher intracellular transfection and gene expression in U87 cells than Lipofectamine-2000.[N,N′-Bis(oleoylcysteinyl)(β-alanyl-α-lysyl)]-spermine monoamide (SKACO) resulted in the highest luciferase transfection efficiency,more than 400 times higher than that of Lipofectamine-2000,and GFP expression in 71% of transfected U87 *** was identified as a promising lead carrier for efficient gene *** spermine-based pH-sensitive amphiphilic carriers have a potential to be further developed as efficient non-viral multifunctional gene delivery systems.

主 题 词：非病毒基因载体 pH敏感 传递系统 多功能 双亲性 精胺 设计 基础 

学科分类：0710[理学-生物科学类] 081704[081704] 07[理学] 08[工学] 0817[工学-轻工类] 09[农学] 071007[071007] 070303[070303] 0703[理学-化学类] 0901[农学-植物生产类] 0836[0836] 090102[090102] 

核心收录：

D　O　I：10.1007/s11426-010-4198-2

馆 藏 号：203426253...