A Survey on the Methods of Primer Design Among Plant Pathologists in Australia and New Zealand

作     者：Francisco M. Ochoa Corona1 Brendan Rodoni Joe Tang 

作者机构：Oklahoma State University National Institute for Microbial Forensics & Food and Agricultural Biosecurity (NIMFFAB) 127Noble Research Center Stillwater OK 74078-3003 USA Department of Primary Industries Knoxfield Centre Private Bag 15 Ferntree Gully Delivery Centre Victoria Australia Plant Health and Environment Laboratory Investigation and Diagnostic Centre MAF Biosecurity New Zealand P.O. Box 2095Auckland 1140 New Zealand 

出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)

年 卷 期：2012年第6卷第5期

页      码：476-480页

摘      要：Designing primers for PCR-based diagnostics was achieved by executing sight searches on DNA sequences. Visual searching for specific DNA targets is time consuming, subjective and requires optimisation among numerous candidate primer sets. Several primer design software have been linked to useful bioinformatic packages to speed the development of PCR assays. Despite the software options available, primer design has remained a challenging aspect of incursion responses, biosecurity emergencies and microbial forensic applications. Two surveys were conducted among 45 plant virologists and 21 other plant pathologists during the 7th Australasian Plant Virology Workshop and the 16th Biennial Australasian Plant Pathology Conference in 2006 and 2007, respectively. Results show that most primer design learning occurs scientist to scientist rather than during academic teaching. This tendency matches with 16% of scientists users of PCR, who do not engage in primer design and 25% designing primers only by visual means, combining a pool of 41% who if trained, would likely enhance their performance in primer design. Only 13 out of 58 scientists ranked themselves as experts. Implementing primer design in study programs and regional training will benefit plant pathology and entomology, and the responsiveness and performance of biosecurity and microbial forensics in the South Pacific.

主 题 词：Primer design PCR education training biosecurity microbial forensics plant pathology. 

学科分类：0710[理学-生物科学类] 07[理学] 09[农学] 071007[071007] 0904[农学-动物医学类] 090401[090401] 

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