Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma

作     者：Genoveva MorraI-Ruiz Pedro Melgar-Lesmes Andrea Lopez-Vicente Conxita Solans Maria Jose Garcia-Celma 

作者机构：Department of Pharmacy and Pharmaceutical Technology Faculty of Pharmacy University of Barcelona Av Joan XXIII s/n 08028 Barcelona Spain Institute of Advanced Chemistry of Catalonia (IQAC) CS/C Jordi Girona 18-26 08034 Barcelona Spain Networking Research Center on Bioengineering Biomaterials and Nanomedicine CIBER-BBN Spain 

基　　金：sponsorship of the Spanish Ministry of Education and Science, DGI “Generalitat de Catalunya” DURSI CIBER-BBN.CIBERBBN is an initiative funded by the VI National R&D&i Plan 2008-2011,Iniciativa Ingenio 2010,Consolider Program,CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fundation 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2015年第8卷第5期

页      码：1729-1745页

摘      要：Over the past years, significant efforts have been devoted to explore novel drug delivery and detection strategies for simultaneous therapy and diagnostics. The development of biotinylated polyurethane-urea nanoparticles as theranostic nanocarriers for targeted drug and plasmid delivery, for fluorescence detection of human hepatocellular carcinoma cells, is described herein. These targeted nanoparticles are specifically designed to incorporate biotin into the polymeric matrix, since many tumor types overexpress receptors for biotin as a mechanism to boost uncontrolled cell growth. The obtained nanoparticles were spherical, exhibited an average diameter ranging 110-145 nm, and showed no cytotoxicity in healthy endothelial cells. Biotinylated nanoparticles are selectively incorporated into the perinuclear and nuclear area of the human hepatocellular carcinoma cell line, HepG2, in division, but not into growing, healthy, human endothelial cells. Indeed, the simultaneous incorporation of the anticancer drugs, phenoxodiol or sunitinib, together with plasmid DNA encoding green fluorescent protein, into these nanoparticles allows a targeted pharmacological antitumor effect and furthermore, selective transfection of a reporter gene, to detect these cancer cells. The combined targeted therapy and detection strategy described here could be exploited for liver cancer therapy and diagnostics, with a moderate safety profile, and may also be a potential tool for other types of cancer.

主 题 词：cancer therapy DNA nanoparticles polyurethane theranostics 

学科分类：071011[071011] 0710[理学-生物科学类] 071010[071010] 07[理学] 

核心收录：

D　O　I：10.1007/s12274-014-0678-6

馆 藏 号：203428365...