Fabrication of a Novel Hybrid Scaffold for Tissue Engineered Heart Valve

作     者：洪昊 董念国 史嘉玮 陈思 郭超 胡平 齐宏旭 Hao HONG1,Nianguo DONG1,Jiawei SHI1,Si CHEN1,Chao GUO1,Ping HU2,Hongxu QI2 1Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China 2Department of Chemical Engineering,Institute of Polymer Science and Technology,Tsinghua University,Beijing 100084,China

作者机构：Department of Cardiovascular SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Chemical EngineeringInstitute of Polymer Science and TechnologyTsinghua University 

基　　金：supported by grants from National Natural Sciences Foundation of China (No.30571839,30600608 and 30872540) National High Technology Research and Development Program ("863" Program) of China (No.2009AA-03Z420) 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2009年第29卷第5期

页      码：599-603页

摘      要：The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue engineered heart valves were engineered by seeding mesenchymal stromal cells （MSCs） onto the scaffolds. The effects of the hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized, coated with poly（3-hydroxybutyrate-co-4-hydroxybutyrate） using an electrospinning technique, and reseeded and cultured over a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent time period. Specimens of each group were examined histologically （hematoxylin-eosin [HE] staining, immunohistostaining, and scanning electron microscopy）, biochemically （DNA and 4-hydroxyproline） and mechanically. The results showed that recellularization was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed comparable amounts of cell mass and 4-hydroxyproline （P〉0.05）. However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls （P〈0.05）. This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction of tissue engineered heart valves.

主 题 词：tissue engineered heart valve hybrid scaffold electrospinning mesenchymal stem cells 

学科分类：0831[工学-公安技术类] 08[工学] 0836[0836] 

核心收录：

D　O　I：10.1007/s11596-009-0513-6

馆 藏 号：203502660...