RNA interference-mediated gene silencing of vascular endothelial growth factor in colon cancer cells

作     者：Tie-Jun Li Jian-Ning Song Kai Kang Shu-Sheng Tong Zan-Lan Hu Tong-Chuan He Bing-Qiang Zhang Cai-Quan Zhang 

作者机构：The First Affiliated Hospital Chongqing University of MedicalSciences Chongqing 400016 China The Ninth Hospital of Chongqing Chongqing 400700 China Molecular Oncology Laboratory University of Chicago Medical Center IL 60637 United States 

基　　金：Supported in part by research grants from the National Natural Science Foundation of China,No.30300298 the National Natural Science Foundation of China's Joint Research Fund for Overseas Chinese Young Scholars,No.30228026 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2007年第13卷第40期

页      码：5312-5316页

摘      要：AIM： TO inhibit the expression of vascular endothelial growth factor （VEGF） in colon cancer cell line by RNA interference （RNAi）. METHODS： Followed the service of E-RNAi, we designed and constructed two kinds of shRNA expression vectors aiming at the VEGF gene, then transfected them into colon cancer HT29 cells by lipofectamineTM 2000. The level of VEGF mRNA was investigated by RT-PCR and Northern blotting. The protein expression of VEGF was observed by immunofluoresence staining and Western blotting. RESULTS： We got two kinds of VEGF specific shRNA expression vectors which could efficiently inhibit the expression of VEGF in HT29 cells. RT-PCR, Northern blotting, immunofluoresence staining and Western blotting showed that inhibition rate for VEGF expression was up to 42%, 89%, 73% and 82%, respectively. CONCLUSION： The expression of VEGF can be inhibited by RNA interference in HT29 cells.

主 题 词：RNA interference Vascular endothelialgrowth factor Colon cancer Northern blotting Western blotting 

学科分类：1002[医学-临床医学类] 100214[100214] 10[医学] 

核心收录：

D　O　I：10.3748/wjg.v13.i40.5312

馆 藏 号：203563264...